Document Detail


Hydroalcoholic human placental extract: skin pigmenting activity and gross chemical composition.
MedLine Citation:
PMID:  7896492     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Vitiligo is a pigmentary disorder of the skin of unknown etiology. It is thought to be of autoimmune origin after demonstration of antibody-mediated destruction of melanocytes. Photochemotherapeutic PUVA therapy is widely used in vitiligo with about 33% success. Aqueous or hydroalcoholic extracts of human placenta of ill-defined composition have also been used therapeutically for vitiligo. A hydroalcoholic human placental extract has been developed by us with pigmenting activity based on experimental therapies. Its chemical analysis was the primary objective of this study. METHODS: For the guinea pig experiment, 20 drops of the extract or vehicle (60% alcohol) as control was topically applied around the nipples covering the areola zones of male immature white guinea pigs (wt. 175-250 g) daily for 60 days with 15 minutes infrared (IR) exposure used for vascular dilatation and enhancement of the absorption of the extract. Standard methods have been followed for all chemical analyses. RESULTS: The guinea pig experiment showed clear pigmentation and hypertrophy of the experimental nipples to varying degrees. Chemical analysis of the extract revealed the presence of small-molecular-weight proteins/peptides, lipids (including glycosphingolipids), carbohydrates, sialic acids, cholesterol, triglycerides, high density lipoproteins (HDL), and others, including amino acids, nucleotides, carotenes, vitamins, etc. CONCLUSION: Glycosphingolipids, known modulators of B and T cells, were reported capable of inducing adhesion, spreading, and motility of melanoma. It is present in the extract and, therefore, may lead to skin pigmentation through induction of melanocytes. Endothelin, a 21-amino acid peptide, detected in human placenta and possibly extractable by our process, has been reported to be indispensable for melanocyte growth.
Authors:
P Pal; R Roy; P K Datta; A K Dutta; B Biswas; R Bhadra
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of dermatology     Volume:  34     ISSN:  0011-9059     ISO Abbreviation:  Int. J. Dermatol.     Publication Date:  1995 Jan 
Date Detail:
Created Date:  1995-04-27     Completed Date:  1995-04-27     Revised Date:  2010-03-24    
Medline Journal Info:
Nlm Unique ID:  0243704     Medline TA:  Int J Dermatol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  61-6     Citation Subset:  IM    
Affiliation:
Department of Cellular Biochemistry, Indian Institute of Chemical Biology, Calcutta.
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MeSH Terms
Descriptor/Qualifier:
Absorption
Administration, Topical
Amino Acids / analysis
Animals
Carotenoids / analysis
Ethanol
Glycolipids / analysis
Glycosphingolipids / analysis
Guinea Pigs
Humans
Infrared Rays
Lipids / analysis
Male
Nipples / drug effects
Phospholipids / analysis
Phototherapy
Placental Extracts / chemistry*,  pharmacokinetics,  pharmacology*,  radiation effects
Proteins / analysis
Sialic Acids / analysis
Skin Pigmentation / drug effects*
Sphingolipids / analysis
Vasodilation / radiation effects
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Glycolipids; 0/Glycosphingolipids; 0/Lipids; 0/Phospholipids; 0/Placental Extracts; 0/Proteins; 0/Sialic Acids; 0/Sphingolipids; 36-88-4/Carotenoids; 64-17-5/Ethanol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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