Document Detail


Hydraulic conductivity, albumin reflection and diffusion coefficients of pig mediastinal pleura.
MedLine Citation:
PMID:  10458927     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hydraulic conductivity (L), albumin reflection coefficient (sigma), and albumin diffusion coefficient (D) were measured across pig mediastinal pleura. The tissue (7 mm diameter) was bonded between two chambers. Flow (Q) of lactated Ringer solution between the chambers was measured in turn at driving pressures (DeltaP) of 2, 4, and 6 cm H(2)O. Value of L was proportional to the slope of the Q-DeltaP curve. Then Q was measured in turn at three albumin osmotic pressure differences (Deltapi equivalent to -1, -2, and -3 g/dl albumin concentration difference, DeltaC) with DeltaP constant at either 2, 3, 4, or 6 cm H(2)O. From Starling's equation, magnitude of sigma was the slope of the Q-Deltapi curve divided by the slope of the Q-DeltaP curve. We measured the diffusion of 0, 2, 5, and 10 g/dl albumin with tracer (125)I-albumin. Tracer mass (M) that diffused across the pleura was measured for 10 h using a well-type NaI(T1) detector. D was calculated from the slope of the M-time curve. Values of L averaged 2.0 x 10(-8) cm(3). s(-1). dyne(-1) (n = 23). Values of sigma were small (0.02-0.05) and sigma increased as flow increased 20-fold. D (n = 24) increased 3-fold from 2.7 x 10(-8) cm(2)/s as DeltaC increased from 0 to 10 g/dl. The small values of sigma indicated that mediastinal pleura provided little restriction to the passage of protein.
Authors:
S Parameswaran; L V Brown; G S Ibbott; S J Lai-Fook
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Microvascular research     Volume:  58     ISSN:  0026-2862     ISO Abbreviation:  Microvasc. Res.     Publication Date:  1999 Sep 
Date Detail:
Created Date:  1999-10-18     Completed Date:  1999-10-18     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0165035     Medline TA:  Microvasc Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  114-27     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Academic Press.
Affiliation:
Center for Biomedical Engineering, University of Kentucky, Lexington, Kentucky 40506-0070, USA.
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MeSH Terms
Descriptor/Qualifier:
Albumins / physiology*
Animals
Biophysical Phenomena
Biophysics
Diffusion
Hydrostatic Pressure
Mediastinum
Microcirculation / physiology
Models, Biological
Osmotic Pressure
Pleura / blood supply*,  physiology*
Swine
Grant Support
ID/Acronym/Agency:
HL 36597/HL/NHLBI NIH HHS; HL 40362/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Albumins

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