Document Detail

Hydration dependence of active core fluctuations in bacteriorhodopsin.
MedLine Citation:
PMID:  18339747     Owner:  NLM     Status:  MEDLINE    
We used neutron scattering and specific hydrogen-deuterium labeling to investigate the thermal dynamics of isotope-labeled amino acids and retinal, predominantly in the active core and extracellular moiety of bacteriorhodopsin (BR) in the purple membrane and the dynamical response to hydration. Measurements on two neutron spectrometers allowed two populations of motions to be characterized. The lower amplitude motions were found to be the same for both the labeled amino acids and retinal of BR and the global membrane. The larger amplitude dynamics of the labeled part, however, were found to be more resilient than the average membrane, suggesting their functional importance. The response to hydration was characterized, showing that the labeled part of BR is not shielded from hydration effects. The results suggest that the inhibition of high-amplitude motions by lowering hydration may play a key role in the slowing down of the photocycle and the proton pumping activity of BR.
Kathleen Wood; Ursula Lehnert; Brigitte Kessler; Giuseppe Zaccai; Dieter Oesterhelt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-13
Journal Detail:
Title:  Biophysical journal     Volume:  95     ISSN:  1542-0086     ISO Abbreviation:  Biophys. J.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-06-13     Completed Date:  2008-07-10     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  194-202     Citation Subset:  IM    
Institut Laue-Langevin, BP 156, F-38042 Grenoble cedex 9, France.
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MeSH Terms
Bacteriorhodopsins / chemistry*,  ultrastructure*
Computer Simulation
Deuterium Exchange Measurement
Models, Chemical*
Models, Molecular*
Neutron Diffraction
Protein Conformation
Purple Membrane / chemistry*,  ultrastructure*
Water / chemistry*
Reg. No./Substance:
53026-44-1/Bacteriorhodopsins; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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