| Hydration dependence of active core fluctuations in bacteriorhodopsin. | |
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MedLine Citation:
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PMID: 18339747 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We used neutron scattering and specific hydrogen-deuterium labeling to investigate the thermal dynamics of isotope-labeled amino acids and retinal, predominantly in the active core and extracellular moiety of bacteriorhodopsin (BR) in the purple membrane and the dynamical response to hydration. Measurements on two neutron spectrometers allowed two populations of motions to be characterized. The lower amplitude motions were found to be the same for both the labeled amino acids and retinal of BR and the global membrane. The larger amplitude dynamics of the labeled part, however, were found to be more resilient than the average membrane, suggesting their functional importance. The response to hydration was characterized, showing that the labeled part of BR is not shielded from hydration effects. The results suggest that the inhibition of high-amplitude motions by lowering hydration may play a key role in the slowing down of the photocycle and the proton pumping activity of BR. |
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Authors:
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Kathleen Wood; Ursula Lehnert; Brigitte Kessler; Giuseppe Zaccai; Dieter Oesterhelt |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-03-13 |
Journal Detail:
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Title: Biophysical journal Volume: 95 ISSN: 1542-0086 ISO Abbreviation: Biophys. J. Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-06-13 Completed Date: 2008-07-10 Revised Date: 2013-06-05 |
Medline Journal Info:
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Nlm Unique ID: 0370626 Medline TA: Biophys J Country: United States |
Other Details:
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Languages: eng Pagination: 194-202 Citation Subset: IM |
Affiliation:
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Institut Laue-Langevin, BP 156, F-38042 Grenoble cedex 9, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacteriorhodopsins
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chemistry*,
ultrastructure* Computer Simulation Deuterium Exchange Measurement Models, Chemical* Models, Molecular* Motion Neutron Diffraction Porosity Protein Conformation Purple Membrane / chemistry*, ultrastructure* Water / chemistry* |
| Chemical | |
Reg. No./Substance:
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53026-44-1/Bacteriorhodopsins; 7732-18-5/Water |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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