| Hybridoma cultivation in defined serum-free media: growth-supporting substances. III. Ethanolamine, vitamins, and other low molecular weight nutrients. | |
| | |
MedLine Citation:
|
PMID: 3803623 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The effects of two low molecular weight substances, ethanolamine and ascorbic acid, on growth of hybridomas in serum-free medium were studied. The optimum growth-supporting concentration of ethanolamine was 20 microM. Omission of ethanolamine led to significantly decreased growth of hybridoma cells (41-75% of the cells growing 3 days with ethanolamine). The optimum growth-supporting concentration of ascorbic acid was 3 micrograms/ml. Omission of ascorbic acid also resulted in decreased growth (60-80% of the cells growing 3 days with ascorbic acid). Other low molecular weight substances, such as 2-mercaptoethanol, putrescine, alpha-tocopherol, and retinal, had no growth-supporting effect. |
| | |
Authors:
|
J Kovár |
Related Documents
:
|
18587903 - Mixed substrate-metabolism in an obligate methanol utilizer: chemostat studies. 3535283 - Assessment of the in vitro growth of plasmodium falciparum using fluorometry. 12640113 - Retinoic acid repression of bone morphogenetic protein 4 in inner ear development. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Folia biologica Volume: 32 ISSN: 0015-5500 ISO Abbreviation: Folia Biol. (Praha) Publication Date: 1986 |
Date Detail:
|
Created Date: 1987-03-03 Completed Date: 1987-03-03 Revised Date: 2000-12-18 |
Medline Journal Info:
|
Nlm Unique ID: 0234640 Medline TA: Folia Biol (Praha) Country: CZECHOSLOVAKIA |
Other Details:
|
Languages: eng Pagination: 369-76 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Ascorbic Acid
/
pharmacology Cells, Cultured Culture Media Dose-Response Relationship, Drug Ethanolamines / pharmacology* Hybridomas / cytology* Mercaptoethanol / pharmacology Putrescine / pharmacology Retinaldehyde / pharmacology Vitamin E / pharmacology Vitamins / pharmacology* |
| Chemical | |
Reg. No./Substance:
|
0/Culture Media; 0/Ethanolamines; 0/Vitamins; 110-60-1/Putrescine; 116-31-4/Retinaldehyde; 1406-18-4/Vitamin E; 50-81-7/Ascorbic Acid; 60-24-2/Mercaptoethanol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Use of in vivo kinetic techniques to evaluate metabolism of nutrients.
Next Document: Detection of antibodies to hepatitis B surface antigen (HBsAg) using monoclonal antibody and the avi...