Document Detail

Hybridoma cell behaviour in continuous culture under hyperosmotic stress.
MedLine Citation:
PMID:  19003339     Owner:  NLM     Status:  PubMed-not-MEDLINE    
In this paper, we propose an alternative strategy to the ones proposed before (Oh et al., 1993; Øyaas et al., 1994a) to get real increases of global final antibody titer and production at hyperosmotic stress, by reducing the detrimental effect of such a stress on cell growth, and conserving the stimulating effect on antibody production. It consists of cultivating the cells in continuous culture and increasing the osmolality stepwise. In this way, the cells could progressively adapt to the higher osmolality at each step and antibody titers could be nearly doubled at 370 and 400 mOsm kg-1, compared to the standard osmolality of 335 mOsm kg-1. Surprisingly, the stimulation of antibody production was not confirmed for higher osmolalities, 425 and 450 mOsm kg- 1, despite the minor negative effect on cell growth. Intracellular IgG analysis by flow cytometry revealed at these osmolalities a significant population of non-producing cells. However, even when taking into account this non-producing population, a stimulating effect on antibody production could not be shown at these highest osmolalities. It seems to us that osmolality has a significant effect on the appearance of these non-producing cells, since they were not observed in continuous cultures at standard osmolality, of comparable duration and at an even higher dilution rate. The appearance of the non-producing cells coincides furthermore with modifications of the synthesised antibody, as shown by electrophoretic techniques. It is however not really clear if these two observations reflect actually the same phenomenon. Hyperosmolality affects the cell behaviour in continuous culture in multiple ways, independently of the growth rate, counting all at least partially for the observed stimulation of antibody production: acceleration of the amino acid, and in particular the glutamine metabolism, increase of the cell volume, increase of the intracellular pH and accumulation of cells in the G1 cell cycle phase.
M Cherlet; A Marc
Related Documents :
16284659 - Validation of new microvolume couette flow linear dichroism cells.
14766949 - Seawater acclimation causes independent alterations in na+/k+- and h+-atpase activity i...
6707729 - Effects of sensory deprivation on the developing mouse olfactory system: a light and el...
16098519 - Mammalian nhe2 na(+)/h+ exchanger mediates efflux of potassium upon heterologous expres...
6255789 - Stereological analysis of the guinea pig adrenal: effects of dexamethasone and acth tre...
1336929 - Intracellular ph regulation by a na+/h+ exchanger in cultured bovine trabecular cells.
3667379 - Effect of tumor colony definition on ionizing radiation survival curves of melanoma-col...
23047589 - Visible 532 nm laser irradiation of human adipose tissue-derived stem cells: effect o...
22812189 - Pik3ca is critical for the proliferation, invasiveness, and drug resistance of human to...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytotechnology     Volume:  29     ISSN:  0920-9069     ISO Abbreviation:  Cytotechnology     Publication Date:  1999 Jan 
Date Detail:
Created Date:  2008-11-12     Completed Date:  2011-07-14     Revised Date:  2013-03-27    
Medline Journal Info:
Nlm Unique ID:  8807027     Medline TA:  Cytotechnology     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  71-84     Citation Subset:  -    
Laboratoire des Sciences du Génie Chimique, CNRS-INPL, BP 451, F-54001, Nancy Cedex, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Preparation of monoclonal antibody against crocin and its characterization.
Next Document:  Estimation of rates of oxygen uptake and carbon dioxide evolution of animal cell culture using mater...