Document Detail

Hybridoma Ped-2E9 cells cultured under modified conditions can sensitively detect Listeria monocytogenes and Bacillus cereus.
MedLine Citation:
PMID:  17043821     Owner:  NLM     Status:  MEDLINE    
Lymphocyte origin hybridoma Ped-2E9 cell-based cytotoxicity assay can detect virulent Listeria or Bacillus species, and its application in a cell-based biosensor for onsite use would be very attractive. However, maintaining enough viable cells on a sensor platform for a prolonged duration is a challenging task. In this study, key factors affecting the survival and growth of Ped-2E9 cells under modified conditions were investigated. When the Ped-2E9 cells were grown in media containing 5% fetal bovine serum in sealed tubes without any replenishment of nutrients or exogenous CO(2) supply, a large portion of the cells remained viable for 6 to 7 days and cells entered into G0/G1 resting phase. The media pH change was negligible and no cell death was observed in the first 4 days, then cells sequentially underwent apoptotic (fourth day onward) phase until day 7 after which a majority was dead. Subsequent cytotoxicity testing of 3- to 7-day stored Ped-2E9 cells sensitively detected virulent Listeria and Bacillus species. These data strongly suggest that Ped-2E9 cells can be maintained in viable state for 6 days in a sealed tube mimicking the environment in a potential sensor device for onsite use without the need for expensive cell culture facilities.
Pratik Banerjee; Mark T Morgan; Jenna L Rickus; Kathy Ragheb; Carlos Corvalan; J Paul Robinson; Arun K Bhunia
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2006-10-17
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  73     ISSN:  0175-7598     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2007-01-11     Completed Date:  2007-05-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1423-34     Citation Subset:  IM    
Molecular Food Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, IN 47907, USA.
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MeSH Terms
Apoptosis / drug effects
Bacillus cereus / growth & development,  metabolism*
Bacterial Toxins / metabolism,  pharmacology
Biosensing Techniques / methods*
Carbon Dioxide / metabolism
Cell Cycle / drug effects
Cell Line
Cell Survival / drug effects
DNA / metabolism
Hybridomas / cytology*,  drug effects,  microbiology
Hydrogen-Ion Concentration
Listeria monocytogenes / growth & development,  metabolism*
Time Factors
Reg. No./Substance:
0/Bacterial Toxins; 124-38-9/Carbon Dioxide; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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