Document Detail


Hybridocytochemical detection of mRNA for calcitonin, CGRP, NPY and somatostatin in thyroid parafollicular (C) cells in three rodent species.
MedLine Citation:
PMID:  12056636     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study was aimed at hybridocytochemical (HCC) detection and interspecies comparison of mRNA for calcitonin (CT), calcitonin gene-related peptide (CGRP), neuropeptide Y (NPY) and somatostatin (SS) in thyroid C cells of two rodent families of wild Microtidae: pine voles and common voles and also of laboratory Muridae, Wistar rats. Studies were performed on adult males. The HCC method in situ and immunomax technique were used to detect mRNA. DNA oligonucleotide probes labeled with digoxigenin were used in the HCC method. The obtained results were compared to the results of immunocytochemical (ICC) examinations, where rabbit or mouse antibodies against human CT, SS, NPY and rat CGRP, as well as chromogranin A were performed. In the present study, HCC reaction has demonstrated the presence of mRNA for CT and CGRP in all thyroid C cells in all the species examined. However, mRNA for NPY and SS was observed in very few C cells in rat and in many more C cells in the two species of wild rodents. The distribution of the positive cells corresponded with that of ICC detected cells.
Authors:
B Sawicki; E Czykier; I Kasacka; A Kasprzak; M Zabel
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society     Volume:  40     ISSN:  0239-8508     ISO Abbreviation:  Folia Histochem. Cytobiol.     Publication Date:  2002  
Date Detail:
Created Date:  2002-06-11     Completed Date:  2003-01-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8502651     Medline TA:  Folia Histochem Cytobiol     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  183-4     Citation Subset:  IM    
Affiliation:
Department of Histology and Embryology, Medical Academy, Białystok, Poland. sawboghe@amb.edu.pl
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MeSH Terms
Descriptor/Qualifier:
Animals
Arvicolinae
Calcitonin / metabolism*
Calcitonin Gene-Related Peptide / metabolism*
Immunohistochemistry
Male
Neuropeptide Y / metabolism*
RNA, Messenger / biosynthesis*,  chemistry
Rats
Rats, Wistar
Somatostatin / metabolism*
Species Specificity
Staining and Labeling
Thyroid Gland / cytology,  metabolism*
Chemical
Reg. No./Substance:
0/Neuropeptide Y; 0/RNA, Messenger; 51110-01-1/Somatostatin; 83652-28-2/Calcitonin Gene-Related Peptide; 9007-12-9/Calcitonin

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