| Hybridization of nucleic acids directly in agarose gels. | |
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MedLine Citation:
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PMID: 6193737 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nucleic acids, both DNA and RNA, separated on agarose gels can be visualized by direct hybridization of the dried gel with appropriate radioactive probes. This method does not involve the transfer of the nucleic acid from the gel. The method requires less manipulation than other procedures; it is extremely rapid, sensitive, and inexpensive. These attributes make this procedure a valuable alternative or supplement to the commonly used methods for visualization by hybridization of nucleic acids separated on agarose gels. |
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Authors:
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S G Tsao; C F Brunk; R E Pearlman |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Analytical biochemistry Volume: 131 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1983 Jun |
Date Detail:
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Created Date: 1983-10-21 Completed Date: 1983-10-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 365-72 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacteriophages
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analysis DNA / isolation & purification* Electrophoresis, Agar Gel / methods Molecular Weight Nucleic Acid Hybridization* Plasmids RNA / isolation & purification* |
| Chemical | |
Reg. No./Substance:
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63231-63-0/RNA; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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