| Hybrid recombinant human leukocyte interferon inhibits differentiation in murine B-16 melanoma cells. | |
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MedLine Citation:
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PMID: 6704115 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have investigated the effects of recombinant human leukocyte interferons (IFN-alpha A and IFN-alpha D) and various hybrid recombinant human leukocyte interferons on differentiation in B-16 mouse melanoma cells. Inhibition of both spontaneous and melanocyte hormone stimulated differentiation was observed with one hybrid construct, IFN-alpha A/D (Bgl) consisting of amino acids 1 to 62 from IFN-alpha A and amino acids 64 to 166 from IFN-alpha D. In contrast, the parental human interferons, IFN-alpha A and IFN-alpha D, when used alone or in combination, as well as other hybrid human leukocyte interferons, did not cause significant inhibition of melanogenesis in B-16 mouse cells. The tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) also inhibited B-16 differentiation and the combination of TPA with IFN-alpha A/D (Bgl) or mouse L-cell interferon was synergistic in delaying melanogenesis. These studies indicate that the IFN-alpha A/D (Bgl) hybrid that exhibits antiviral activity on mouse cells can also inhibit differentiation of murine cells. |
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Authors:
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P B Fisher; H Hermo; D R Prignoli; I B Weinstein; S Pestka |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 119 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1984 Feb |
Date Detail:
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Created Date: 1984-04-26 Completed Date: 1984-04-26 Revised Date: 2008-11-24 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 108-15 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Differentiation / drug effects Cell Line DNA, Recombinant* Interferon Type I / genetics, pharmacology* L Cells (Cell Line) / analysis Melanins / biosynthesis Melanocyte-Stimulating Hormones / pharmacology Melanoma / metabolism, pathology* Mice Tetradecanoylphorbol Acetate / pharmacology Two-Hybrid System Techniques |
| Chemical | |
Reg. No./Substance:
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0/DNA, Recombinant; 0/Interferon Type I; 0/Melanins; 16561-29-8/Tetradecanoylphorbol Acetate; 9002-79-3/Melanocyte-Stimulating Hormones |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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