Document Detail


Hybrid gel composed of native heart matrix and collagen induces cardiac differentiation of human embryonic stem cells without supplemental growth factors.
MedLine Citation:
PMID:  21744185     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Our goal was to assess the ability of native heart extracellular matrix (ECM) to direct cardiac differentiation of human embryonic stem cells (hESCs) in vitro. In order to probe the effects of cardiac matrix on hESC differentiation, a series of hydrogels was prepared from decellularized ECM from porcine hearts by mixing ECM and collagen type I at varying ratios. Maturation of cardiac function in embryoid bodies formed from hESCs was documented in terms of spontaneous contractile behavior and the mRNA and protein expression of cardiac markers. Hydrogel with high ECM content (75% ECM, 25% collagen, no supplemental soluble factors) increased the fraction of cells expressing cardiac marker troponin T, when compared with either hydrogel with low ECM content (25% ECM, 75% collagen, no supplemental soluble factors) or collagen hydrogel (100% collagen, with supplemental soluble factors). Furthermore, cardiac maturation was promoted in high-ECM content hydrogels, as evidenced by the striation patterns of cardiac troponin I and by upregulation of Cx43 gene. Consistently, high-ECM content hydrogels improved the contractile function of cardiac cells, as evidenced by increased numbers of contracting cells and increased contraction amplitudes. The ability of native ECM hydrogel to induce cardiac differentiation of hESCs without the addition of soluble factors makes it an attractive biomaterial system for basic studies of cardiac development and potentially for the delivery of therapeutic cells into the heart.
Authors:
Yi Duan; Zen Liu; John O'Neill; Leo Q Wan; Donald O Freytes; Gordana Vunjak-Novakovic
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-07-09
Journal Detail:
Title:  Journal of cardiovascular translational research     Volume:  4     ISSN:  1937-5395     ISO Abbreviation:  J Cardiovasc Transl Res     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-09-19     Completed Date:  2012-01-12     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  101468585     Medline TA:  J Cardiovasc Transl Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  605-15     Citation Subset:  IM    
Affiliation:
Department of Biomedical Engineering, Columbia University, New York, NY 10032, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers / metabolism
Cell Differentiation*
Cells, Cultured
Coculture Techniques
Collagen Type I / metabolism*
Connexin 43 / metabolism
Embryonic Stem Cells / metabolism*,  transplantation
Extracellular Matrix / metabolism*
Humans
Hydrogels
Myocardial Contraction
Myocytes, Cardiac / metabolism*,  transplantation
Regenerative Medicine / methods*
Swine
Time Factors
Tissue Engineering*
Tissue Scaffolds*
Troponin I / metabolism
Troponin T / genetics
Up-Regulation
Grant Support
ID/Acronym/Agency:
EB002520/EB/NIBIB NIH HHS; HL076485/HL/NHLBI NIH HHS; R01 HL076485/HL/NHLBI NIH HHS; R01 HL076485-06S1/HL/NHLBI NIH HHS; R21 HL108668/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Collagen Type I; 0/Connexin 43; 0/Hydrogels; 0/TNNT2 protein, human; 0/Troponin I; 0/Troponin T
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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