| Hybrid gel composed of native heart matrix and collagen induces cardiac differentiation of human embryonic stem cells without supplemental growth factors. | |
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MedLine Citation:
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PMID: 21744185 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Our goal was to assess the ability of native heart extracellular matrix (ECM) to direct cardiac differentiation of human embryonic stem cells (hESCs) in vitro. In order to probe the effects of cardiac matrix on hESC differentiation, a series of hydrogels was prepared from decellularized ECM from porcine hearts by mixing ECM and collagen type I at varying ratios. Maturation of cardiac function in embryoid bodies formed from hESCs was documented in terms of spontaneous contractile behavior and the mRNA and protein expression of cardiac markers. Hydrogel with high ECM content (75% ECM, 25% collagen, no supplemental soluble factors) increased the fraction of cells expressing cardiac marker troponin T, when compared with either hydrogel with low ECM content (25% ECM, 75% collagen, no supplemental soluble factors) or collagen hydrogel (100% collagen, with supplemental soluble factors). Furthermore, cardiac maturation was promoted in high-ECM content hydrogels, as evidenced by the striation patterns of cardiac troponin I and by upregulation of Cx43 gene. Consistently, high-ECM content hydrogels improved the contractile function of cardiac cells, as evidenced by increased numbers of contracting cells and increased contraction amplitudes. The ability of native ECM hydrogel to induce cardiac differentiation of hESCs without the addition of soluble factors makes it an attractive biomaterial system for basic studies of cardiac development and potentially for the delivery of therapeutic cells into the heart. |
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Authors:
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Yi Duan; Zen Liu; John O'Neill; Leo Q Wan; Donald O Freytes; Gordana Vunjak-Novakovic |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-07-09 |
Journal Detail:
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Title: Journal of cardiovascular translational research Volume: 4 ISSN: 1937-5395 ISO Abbreviation: J Cardiovasc Transl Res Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-09-19 Completed Date: 2012-01-12 Revised Date: 2013-02-08 |
Medline Journal Info:
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Nlm Unique ID: 101468585 Medline TA: J Cardiovasc Transl Res Country: United States |
Other Details:
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Languages: eng Pagination: 605-15 Citation Subset: IM |
Affiliation:
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Department of Biomedical Engineering, Columbia University, New York, NY 10032, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Markers / metabolism Cell Differentiation* Cells, Cultured Coculture Techniques Collagen Type I / metabolism* Connexin 43 / metabolism Embryonic Stem Cells / metabolism*, transplantation Extracellular Matrix / metabolism* Humans Hydrogels Myocardial Contraction Myocytes, Cardiac / metabolism*, transplantation Regenerative Medicine / methods* Swine Time Factors Tissue Engineering* Tissue Scaffolds* Troponin I / metabolism Troponin T / genetics Up-Regulation |
| Grant Support | |
ID/Acronym/Agency:
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EB002520/EB/NIBIB NIH HHS; HL076485/HL/NHLBI NIH HHS; R01 HL076485/HL/NHLBI NIH HHS; R01 HL076485-06S1/HL/NHLBI NIH HHS; R21 HL108668/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Collagen Type I; 0/Connexin 43; 0/Hydrogels; 0/TNNT2 protein, human; 0/Troponin I; 0/Troponin T |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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