Document Detail


Hyaluronic acid-human blood hydrogels for stem cell transplantation.
MedLine Citation:
PMID:  22898181     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tissue engineering-based approaches have the potential to improve stem cell engraftment by increasing cell delivery to the myocardium. Our objective was to develop and characterize a naturally-derived, autologous, biodegradable hydrogel in order to improve acute stem cell retention in the myocardium. HA-blood hydrogels (HA-BL) were synthesized by mixing in a 1:1(v/v) ratio, lysed whole blood and hyaluronic acid (HA), whose carboxyl groups were functionalized with N-hydroxysuccinimide (NHS) to yield HA succinimidyl succinate (HA-NHS). We performed physical characterization and measured survival/proliferation of cardiosphere-derived cells (CDCs) encapsulated in the hydrogels. Hydrogels were injected intra-myocardially or applied epicardially in rats. NHS-activated carboxyl groups in HA react with primary amines present in blood and myocardium to form amide bonds, resulting in a 3D hydrogel bound to tissue. HA-blood hydrogels had a gelation time of 58±12 s, swelling ratio of 10±0.5, compressive and elastic modulus of 14±3 and 1.75±0.6 kPa respectively. These hydrogels were not degraded at 4 wks by hydrolysis alone. CDC encapsulation promoted their survival and proliferation. Intra-myocardial injection of CDCs encapsulated in these hydrogels greatly increased acute myocardial retention (p=0.001). Epicardial application of HA-blood hydrogels improved left ventricular ejection fraction following myocardial infarction (p=0.01). HA-blood hydrogels are highly adhesive, biodegradable, promote CDC survival and increase cardiac function following epicardial application after myocardial infarction.
Authors:
Connie Y Chang; Angel T Chan; Patrick A Armstrong; Hong-Chang Luo; Takahiro Higuchi; Iossif A Strehin; Styliani Vakrou; Xiaoping Lin; Sophia N Brown; Brian O'Rourke; Theodore P Abraham; Richard L Wahl; Charles J Steenbergen; Jennifer H Elisseeff; M Roselle Abraham
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-08-13
Journal Detail:
Title:  Biomaterials     Volume:  33     ISSN:  1878-5905     ISO Abbreviation:  Biomaterials     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-08-31     Completed Date:  2013-01-16     Revised Date:  2014-03-19    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  8026-33     Citation Subset:  IM    
Copyright Information:
Published by Elsevier Ltd.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Cells / chemistry*,  metabolism
Cell Proliferation
Cell Survival
Cells, Cultured
Elastic Modulus
Female
Humans
Hyaluronic Acid / chemistry*,  metabolism
Hydrogels / chemistry*,  metabolism
Male
Myocardial Infarction / pathology,  surgery
Myocardium / cytology*,  pathology
Plasma / chemistry*,  metabolism
Rats
Rats, Inbred WKY
Rats, Nude
Stem Cell Transplantation*
Succinimides / chemistry
Tissue Scaffolds / chemistry*
Grant Support
ID/Acronym/Agency:
5UL1RR025005-05/RR/NCRR NIH HHS; R01 HL039752/HL/NHLBI NIH HHS; R01 HL092985/HL/NHLBI NIH HHS; R01 HL092985/HL/NHLBI NIH HHS; T32HL07227/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Hydrogels; 0/Succinimides; 9004-61-9/Hyaluronic Acid; MJE3791M4T/N-hydroxysuccinimide
Comments/Corrections

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