| Hyaluronic acid-human blood hydrogels for stem cell transplantation. | |
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MedLine Citation:
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PMID: 22898181 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Tissue engineering-based approaches have the potential to improve stem cell engraftment by increasing cell delivery to the myocardium. Our objective was to develop and characterize a naturally-derived, autologous, biodegradable hydrogel in order to improve acute stem cell retention in the myocardium. HA-blood hydrogels (HA-BL) were synthesized by mixing in a 1:1(v/v) ratio, lysed whole blood and hyaluronic acid (HA), whose carboxyl groups were functionalized with N-hydroxysuccinimide (NHS) to yield HA succinimidyl succinate (HA-NHS). We performed physical characterization and measured survival/proliferation of cardiosphere-derived cells (CDCs) encapsulated in the hydrogels. Hydrogels were injected intra-myocardially or applied epicardially in rats. NHS-activated carboxyl groups in HA react with primary amines present in blood and myocardium to form amide bonds, resulting in a 3D hydrogel bound to tissue. HA-blood hydrogels had a gelation time of 58±12 s, swelling ratio of 10±0.5, compressive and elastic modulus of 14±3 and 1.75±0.6 kPa respectively. These hydrogels were not degraded at 4 wks by hydrolysis alone. CDC encapsulation promoted their survival and proliferation. Intra-myocardial injection of CDCs encapsulated in these hydrogels greatly increased acute myocardial retention (p=0.001). Epicardial application of HA-blood hydrogels improved left ventricular ejection fraction following myocardial infarction (p=0.01). HA-blood hydrogels are highly adhesive, biodegradable, promote CDC survival and increase cardiac function following epicardial application after myocardial infarction. |
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Authors:
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Connie Y Chang; Angel T Chan; Patrick A Armstrong; Hong-Chang Luo; Takahiro Higuchi; Iossif A Strehin; Styliani Vakrou; Xiaoping Lin; Sophia N Brown; Brian O'Rourke; Theodore P Abraham; Richard L Wahl; Charles J Steenbergen; Jennifer H Elisseeff; M Roselle Abraham |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-08-13 |
Journal Detail:
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Title: Biomaterials Volume: 33 ISSN: 1878-5905 ISO Abbreviation: Biomaterials Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-08-31 Completed Date: 2013-01-16 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 8026-33 Citation Subset: IM |
Copyright Information:
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Published by Elsevier Ltd. |
Affiliation:
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Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Cells / chemistry*, metabolism Cell Proliferation Cell Survival Cells, Cultured Elastic Modulus Female Humans Hyaluronic Acid / chemistry*, metabolism Hydrogels / chemistry*, metabolism Male Myocardial Infarction / pathology, surgery Myocardium / cytology*, pathology Plasma / chemistry*, metabolism Rats Rats, Inbred WKY Rats, Nude Stem Cell Transplantation* Succinimides / chemistry Tissue Scaffolds / chemistry* |
| Grant Support | |
ID/Acronym/Agency:
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5UL1RR025005-05/RR/NCRR NIH HHS; R01 HL092985/HL/NHLBI NIH HHS; R01 HL092985/HL/NHLBI NIH HHS; T32HL07227/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Hydrogels; 0/Succinimides; 9004-61-9/Hyaluronic Acid; MJE3791M4T/N-hydroxysuccinimide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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