Document Detail


Hyaluronic acid regulates normal intestinal and colonic growth in mice.
MedLine Citation:
PMID:  22556141     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hyaluronic acid (HA), a component of the extracellular matrix, affects gastrointestinal epithelial proliferation in injury models, but its role in normal growth is unknown. We sought to determine the effects of exogenous HA on intestinal and colonic growth by intraperitoneal injection of HA twice a week into C57BL/6 mice from 3 to 8 wk of age. Similarly, to determine the effects of endogenous HA on intestinal and colonic growth, we administered PEP-1, a peptide that blocks the binding of HA to its receptors, on the same schedule. In mice treated with exogenous HA, villus height and crypt depth in the intestine, crypt depth in the colon, and epithelial proliferation in the intestine and colon were increased. In mice treated with PEP-1, intestinal and colonic length were markedly decreased and crypt depth and villus height in the intestine, crypt depth in the colon, and epithelial proliferation in the intestine and colon were decreased. Administration of HA was associated with increased levels of EGF (intestine) and IGF-I (colon), whereas administration of PEP-1 was associated with decreased levels of IGF-I (intestine) and epiregulin (colon). Exogenous HA increases intestinal and colonic epithelial proliferation, resulting in hyperplasia. Blocking the binding of endogenous HA to its receptors results in decreased intestinal and colonic length and a mucosal picture of hypoplasia, suggesting that endogenous HA contributes to the regulation of normal intestinal and colonic growth.
Authors:
Terrence E Riehl; Xueping Ee; William F Stenson
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-05-03
Journal Detail:
Title:  American journal of physiology. Gastrointestinal and liver physiology     Volume:  303     ISSN:  1522-1547     ISO Abbreviation:  Am. J. Physiol. Gastrointest. Liver Physiol.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-08-02     Completed Date:  2012-10-11     Revised Date:  2013-08-14    
Medline Journal Info:
Nlm Unique ID:  100901227     Medline TA:  Am J Physiol Gastrointest Liver Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  G377-88     Citation Subset:  IM    
Affiliation:
Division of Gastroenterology, Washington University, St. Louis, Missouri, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD44 / drug effects
Cell Proliferation / drug effects
Colon / drug effects,  growth & development*
Epidermal Growth Factor / metabolism
Epithelial Cells / cytology
Glucuronosyltransferase / biosynthesis
Hyaluronic Acid / pharmacology*
Hyaluronoglucosaminidase / biosynthesis
Insulin-Like Growth Factor I / metabolism
Intestines / drug effects,  growth & development*
Mice
Mice, Inbred C57BL
Peptides / pharmacology
RNA, Messenger / metabolism
Grant Support
ID/Acronym/Agency:
P30-DK-52574/DK/NIDDK NIH HHS; R01-DK-55753/DK/NIDDK NIH HHS; R37 DK-33165/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD44; 0/Peptides; 0/RNA, Messenger; 0/epiregulin; 0/glycyl-alanyl-histidyl-tryptophyl-glutaminyl-phenylalanyl-asparagyl-alanyl-leucyl-threonyl-valyl-arginine; 62229-50-9/Epidermal Growth Factor; 67763-96-6/Insulin-Like Growth Factor I; 9004-61-9/Hyaluronic Acid; EC 2.4.1.17/Glucuronosyltransferase; EC 2.4.1.212/hyaluronan synthase; EC 3.2.1.35/Hyaluronoglucosaminidase
Comments/Corrections

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