| Hyaluronate degradation affects ventricular function of the early postlooped embryonic rat heart in situ. | |
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MedLine Citation:
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PMID: 8293563 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hyaluronic acid is the major glycosaminoglycan of the early cardiac extracellular matrix or "cardiac jelly," yet little is known about its role in the ontogeny of early ventricular performance. To investigate the in situ effect of hyaluronate degradation on ventricular function, whole rat embryos were cultured in rat serum alone (control embryos) or rat serum plus 20 TRU/mL of Streptomyces hyaluronidase (treatment embryos) from gestational day 9.5 (before formation of the heart tube) through initial looping of the heart. Cardiac function was measured before looping (24 hours in culture) and immediately after looping (36 hours in culture) by video motion analysis of the external wall motion of the bulbus cordis and primitive ventricle. Degradation of hyaluronic acid in the treated embryos was confirmed by Alcian blue staining at pH 2.5. Significant increases in heart rate, circumferential shortening fraction, maximum velocity of circumferential contraction, and maximum velocity of circumferential relaxation were observed with looping in both control and treatment embryos. Although there was minimal difference in ventricular performance between control and treatment embryos before looping, there was a significant increase in all parameters of ventricular performance in the hyaluronidase-treated embryos immediately after looping of the heart. Endocardial cushions were absent in hyaluronidase-treated embryos, and an additional group of embryos cultured in the presence of Streptomyces hyaluronidase for 48 to 72 hours failed to develop endocardial cushions. These experiments are the first to (1) document a quantifiable increase in ventricular performance during early cardiac looping and (2) demonstrate that hyaluronate degradation results in abnormal endocardial cushion formation and altered ventricular performance of the postlooped heart.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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H S Baldwin; T R Lloyd; M Solursh |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Circulation research Volume: 74 ISSN: 0009-7330 ISO Abbreviation: Circ. Res. Publication Date: 1994 Feb |
Date Detail:
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Created Date: 1994-02-28 Completed Date: 1994-02-28 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0047103 Medline TA: Circ Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 244-52 Citation Subset: IM |
Affiliation:
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Department of Pediatrics (Cardiology), University of Pennsylvania/Children's Hospital of Philadelphia 19014. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Embryo, Mammalian / drug effects, metabolism, physiology* Heart / embryology* Histological Techniques Hyaluronic Acid / metabolism* Hyaluronoglucosaminidase / pharmacology Rats Rats, Wistar Reference Values Ventricular Function* |
| Grant Support | |
ID/Acronym/Agency:
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HL-02197/HL/NHLBI NIH HHS; HL-43600/HL/NHLBI NIH HHS; HL-67413/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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9004-61-9/Hyaluronic Acid; EC 3.2.1.35/Hyaluronoglucosaminidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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