| Hyaluronan-modified and regular multilamellar liposomes provide sub-cellular targeting to macrophages, without eliciting a pro-inflammatory response. | |
| | |
MedLine Citation:
|
PMID: 22019559 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Macrophages, pivotal cells in onset and progression of inflammation, can benefit from sub-cellular drug targeting to the molecular loci of drug action, whether cell membrane or cell interior. Postulating manipulation of liposome size and surface properties can provide sub-cellular targeting, we studied: thermodynamics of liposome-macrophage binding; liposome cellular localizations; liposome safety including pro-inflammatory cytokine production. We aimed at extending the body of knowledge on interactions of regular unilamellar (RL-ULV) and multilamellar (RL-MLV) liposomes with macrophages. We investigated, for the first time, the interactions of hyaluronan (HA) surface-modified liposomes (HA-ULV and HA-MLV) with macrophages, with respect to multiple equilibria binding combined with cellular localization. Macrophages bound all four liposome types, substantially-favoring the two MLV species over the two ULV species, and internalizing only RL-MLV. Three macrophage-internalization inhibitors (2-deoxyglucose, LY294002 and Wortmannin) reduced RL-MLV internalization but not binding affinity nor binding capacity. Both MLV types were not detrimental to cell proliferation, nor did they elicit TNF-α production in resting and in LPS-activated macrophages. Moreover, a 24-hour exposure of LPS-activated macrophages to HA-MLV reduced TNF-α production by 40%, indicating potential for anti-inflammatory activity. In conclusion RL-MLV and HA-MLV are the liposomes of choice for delivering anti-inflammatory drugs to the macrophage surface or its interior, according to the loci of drug action. |
| | |
Authors:
|
Yifat Glucksam-Galnoy; Tsaffrir Zor; Rimona Margalit |
Related Documents
:
|
19819699 - Life in the inflamed intestine, salmonella style. 20580439 - Clathrin-mediated endocytosis and transcytosis of enterotoxigenic escherichia coli f4 f... 19491259 - Puma suppresses intestinal tumorigenesis in mice. 16922089 - Intestinal iga synthesis: a primitive form of adaptive immunity that regulates microbia... 15808309 - Development of a porcine small intestinal cdna micro-array: characterization and functi... 21865549 - Genome-wide expression profiling and mutagenesis studies reveal that lipopolysaccharide... 9007149 - The immune system in severe ovarian hyperstimulation syndrome. 9681509 - Transforming growth factor-beta1 induces nuclear to cytoplasmic distribution of androge... 10049769 - Photodynamic therapy-induced apoptosis in lymphoma cells: translocation of cytochrome c... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-10-12 |
Journal Detail:
|
Title: Journal of controlled release : official journal of the Controlled Release Society Volume: - ISSN: 1873-4995 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
|
Created Date: 2011-10-24 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8607908 Medline TA: J Control Release Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011. Published by Elsevier B.V. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Pyridylthiourea-grafted polyethylenimine offers an effective assistance to siRNA-mediated gene silen...
Next Document: Decrease in juvenile hormone level as a result of genetic ablation of the Corpusallatum cells affect...