Document Detail


Humoral detection of leukaemia-associated antigens in presentation acute myeloid leukaemia.
MedLine Citation:
PMID:  16112646     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The serological analysis of recombinant cDNA expression libraries (SEREX) technique was used to immunoscreen a testes cDNA expression library with sera from newly diagnosed acute myeloid leukaemia (AML) patients. We used a testis cDNA library to aid our identification of cancer-testis (CT) antigens. We identified 44 antigens which we further immunoscreened with sera from AML, chronic myeloid leukaemia (CML), and normal donors. Eight antigens were solely recognised by patient sera including the recently described CT antigen, PASD1, and the cancer-related SSX2 interacting protein, SSX2IP. RT-PCR analysis indicated that we had identified three antigens which were expressed in patient bone marrow (BM) and peripheral blood (PB) but not in normal donor samples (PASD1, SSX2IP, and GRINL1A). Real-time PCR (RQ-PCR) confirmed the restricted expression of PASD1 in normal donor organs. Antigen presentation assays using monocyte-derived dendritic cells (mo-DCs) showed that PASD1 could stimulate autologous T-cell responses, suggesting that PASD1 could be a promising target for future immunotherapy clinical trials.
Authors:
Barbara-Ann Guinn; Elizabeth A Bland; Usman Lodi; Amanda P Liggins; Khalid Tobal; Sarah Petters; James W Wells; Alison H Banham; Ghulam J Mufti
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Publication Detail:
Type:  Clinical Trial; Controlled Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  335     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-08-30     Completed Date:  2005-11-14     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1293-304     Citation Subset:  IM    
Affiliation:
Department of Haematological Medicine, Guy's, King's and St. Thomas' School of Medicine, King's College London, The Rayne Institute, 123 Coldharbour Lane, London SE5 9NU, UK. barbara.guinn@kcl.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Antibody Formation / immunology
Antigens, Neoplasm / immunology*
Cells, Cultured
Humans
Leukemia, Myeloid, Acute / immunology*
Male
Middle Aged
Testis / immunology*
Tumor Markers, Biological / immunology*
Chemical
Reg. No./Substance:
0/Antigens, Neoplasm; 0/Tumor Markers, Biological

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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