| Human uterine epithelial RL95-2 and HEC-1A cell-line adhesiveness: the role of plexin B1. | |
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MedLine Citation:
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PMID: 17383649 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To study the expression of plexin-B1 in high- and low-receptive epithelial-endometrial cell lines, and its possible role in endometrial adhesiveness. DESIGN: Controlled, laboratory study. SETTING: Laboratory for Research in Reproductive Sciences, Department of Obstetrics and Gynecology, Ha'Emek Medical Center, Afula, Israel. PATIENT(S): None. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): This study was designed to explore and compare the expression and role of plexin-B1 in endometrial cell lines RL95-2 and HEC-1A, used as models of receptive and nonreceptive cells, respectively. The expression of plexin-B1 was analyzed by Western blotting and reverse-transcriptase polymerase chain reaction. The possible role of plexin-B1 in endometrial-trophoblast adhesiveness was studied with attachment and invasion assays. For further validation, we transfected HEC-1A cells with an expressing vector encoded for plexin-B1. RESULT(S): Significant differences in spheroid attachment was observed between RL95-2 and HEC-1A cells. Western blotting and reverse-transcriptase polymerase chain reaction revealed that in RL95-2 cells, the expression of plexin-B1 was significantly higher. An attachment assay that used RL95-2 cells in the presence of inhibiting antibodies against plexin-B1 significantly decreased the attachment rates of spheroids. A comparison between HEC-1A and transfected HEC-1A (HEC-1A-2) cells showed significant differences in spheroid attachment. No significant difference was found between HEC-1A-2 and RL95-2. An attachment assay using inhibitory antibodies against plexin-B1 significantly decreased the spheroid-attachment rate. CONCLUSION(S): Based on our results, we think that plexin-B1 contributes to trophoblast-endometrium interactions, most likely by enhancing adhesion properties. |
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Authors:
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Haggar Harduf; Shlomit Goldman; Eliezer Shalev |
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Publication Detail:
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Type: Journal Article Date: 2007-03-26 |
Journal Detail:
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Title: Fertility and sterility Volume: 87 ISSN: 1556-5653 ISO Abbreviation: Fertil. Steril. Publication Date: 2007 Jun |
Date Detail:
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Created Date: 2007-06-04 Completed Date: 2007-09-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372772 Medline TA: Fertil Steril Country: United States |
Other Details:
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Languages: eng Pagination: 1419-27 Citation Subset: IM |
Affiliation:
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Laboratory for Research in Reproductive Sciences, Department of Obstetrics and Gynecology, Ha'Emek Medical Center, Afula, Israel. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Adhesion
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physiology* Cell Line Endometrium / physiology Epithelial Cells / cytology, physiology* Female Humans Nerve Tissue Proteins / genetics, physiology* Receptors, Cell Surface / genetics, physiology* Trophoblasts / physiology Uterus / cytology, physiology* |
| Chemical | |
Reg. No./Substance:
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0/Nerve Tissue Proteins; 0/PLXNB1 protein, human; 0/Receptors, Cell Surface |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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