Document Detail


Human urinary bladder cancer T24 cells are susceptible to the Antrodia camphorata extracts.
MedLine Citation:
PMID:  16455193     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bladder cancer has been cited to result from the neoplastic lesion with environmental and/or occupational factors identified as causatives. Transitional cell carcinoma (TCC) is the most common type of bladder cancer. Most of the bladder cancer patients die from the invasive, metastatic TCC that has turned out to be resistant to chemotherapy. T24 cells, a cell line established from a human urinary bladder cancer patient, are high-grade and invasive TCC. T24 cells were found very susceptible to ACCE at concentration of 50 microg/mL. MTT assay showed that the cell growth and proliferation were inhibited to 50% of the control when treated with ACCE for 72 h, at which the cell proliferation suppressing rate revealed -4.4 x 10(3)cells/microg per day. Comparing the expressions of the cell cycle biomarkers Cdc2 and Cyclin B1 by the western blot analysis, a phase G(2)M arrest was confirmed. Both the wound scratch assay and the transwell motility assay indicated that ACCE was very effective anti-metastatic against T24 cells. Furthermore, the active form of matrix metalloproteinase-9 (MMP-9) was also found totally suppressed as revealed by zymography at 72 h post-incubation with ACCE, while the light and electron microscopic images have apparently revealed cell membrane damages on T24 cells when treated with ACCE (50 microg/mL). Moreover, both the wound scratch and the transwell assays have demonstrated the migration capability of T24 cells has been significantly retarded to 1.5-fold at same dosage of ACCE used. In conclusion, ACCE is a good anti-cancer agent, being effective in inducing phase G(2)M arrest, acting as an anti-proliferative, and an anti-metastatic agent against bladder cancer cell T24 cells.
Authors:
Chiung-Chi Peng; Kuan-Chou Chen; Robert Y Peng; Ching-Hua Su; Hsiu Mei Hsieh-Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-02-07
Journal Detail:
Title:  Cancer letters     Volume:  243     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-10-09     Completed Date:  2006-12-06     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  109-19     Citation Subset:  IM    
Affiliation:
Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan, ROC.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents / chemistry,  pharmacology*
Blotting, Western
CDC2 Protein Kinase / metabolism
Carcinoma, Transitional Cell / metabolism,  pathology,  physiopathology
Cell Cycle / drug effects*
Cell Cycle Proteins / metabolism
Cell Division / drug effects
Cell Line, Tumor
Cell Membrane / drug effects,  ultrastructure
Cell Movement / drug effects
Cell Proliferation / drug effects*
Cell Survival / drug effects
Cyclin B / metabolism
Cyclin B1
Dose-Response Relationship, Drug
Flow Cytometry
G2 Phase / drug effects
Humans
Microscopy, Electron, Scanning
Polyporales / chemistry*
Polysaccharides / analysis,  pharmacology
Time Factors
Triterpenes / analysis,  pharmacology
Urinary Bladder Neoplasms / metabolism,  pathology,  physiopathology
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/CCNB1 protein, human; 0/Cell Cycle Proteins; 0/Cyclin B; 0/Cyclin B1; 0/Polysaccharides; 0/Triterpenes; EC 2.7.11.22/CDC2 Protein Kinase

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