Document Detail


Human uridine-cytidine kinase phosphorylation of ribavirin: a convenient method for activation of ribavirin for conjugation to proteins.
MedLine Citation:
PMID:  18080217     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ribavirin is a synthetic nucleoside analog that is used for the treatment of hepatitis C virus (HCV) infection. Its primary toxicity is hemolytic anemia, which sometimes necessitates dose reduction or discontinuation of therapy. Selective delivery of ribavirin into liver cells would be desirable to enhance its antiviral activity and avoid systemic side effects. One approach to liver-specific targeting is conjugation of the ribavirin with asialoglycoprotein that is taken up specifically by liver cells. Human uridine-cytidine kinase-1 (UCK-1) was used for ribavirin phosphorylation to its monophosphate form. 1-Ethyl-3-diisopropylaminocarbodiimide (EDC) was used as a coupling agent. The best results were obtained using direct conjugation protocol with a molar ratio of 6.5 ribavirin monophosphate (RMP) molecules per one asialoorosomucoid (AsOR) molecule. Our findings show that ribavirin is a potential substrate of UCK-1, and RMP formed could be chemically coupled to AsOR to form a conjugate for liver specific targeting.
Authors:
Lucija Virovic Jukic; Marko Duvnjak; Catherine H Wu; George Y Wu
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Publication Detail:
Type:  Journal Article     Date:  2007-12-13
Journal Detail:
Title:  Journal of biomedical science     Volume:  15     ISSN:  1423-0127     ISO Abbreviation:  J. Biomed. Sci.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-02-25     Completed Date:  2008-04-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9421567     Medline TA:  J Biomed Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  205-13     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine, Division of Gastroenterology and Hepatology, Charity Sisters University Hospital, Vinogradska cesta 29, 10 000, Zagreb, Croatia, lucija.virovic@zg.t-com.hr
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MeSH Terms
Descriptor/Qualifier:
Anemia, Hemolytic / chemically induced,  enzymology
Asialoglycoproteins / chemistry*,  therapeutic use
Drug Delivery Systems*
Hepacivirus
Hepatitis C / complications,  enzymology*
Humans
Liver / enzymology*,  virology
Nucleoside-Phosphate Kinase / chemistry*,  metabolism
Orosomucoid / analogs & derivatives*,  chemistry,  therapeutic use
Phosphorylation
Ribavirin / adverse effects,  chemistry*,  therapeutic use
Chemical
Reg. No./Substance:
0/Asialoglycoproteins; 0/Orosomucoid; 0/asialoorosomucoid; 36791-04-5/Ribavirin; EC 2.7.4.14/cytidylate kinase; EC 2.7.4.4/Nucleoside-Phosphate Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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