| Human umbilical mesenchymal stem cell and its adipogenic differentiation: Profiling by nuclear magnetic resonance spectroscopy. | |
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MedLine Citation:
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PMID: 22577495 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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AIM: To study the metabolic profile of human umbilical mesenchymal stem cells (HUMSC) and adipogenic differentiation by nuclear magnetic resonance (NMR) spectroscopy. METHODS: HUMSC isolated from human umbilical cord stroma were induced to adipocytes over 2 wk by adding dexamethasone, 3-isobutyl-1-methylxanthine, indomethacin, and insulin to the culture medium. Adipogenic differentiation was confirmed by Red O staining and transcription-polymerase chain reaction. Perchloric acid extracts of the HUMSCs and adipocytes (about 7 × 10(6)) were characterized for metabolites by using in vitro high resolution 9.4T NMR spectroscopy. RESULTS: Several major metabolites, such as: choline, creatine, glutamate and myo-inositol, acetate, and some fatty acids/triglycerides, were observed in the MR spectroscopic pattern of HUMSCs and their adipogenic differentiation. HUMSCs are characterized by an unusually low number of NMR-detectable metabolites, high choline, acetate, glutamate and creatine content. However, the metabolic profiles of adipogenic differentiation demonstrated considerably higher methionine and fatty acids, and non-detectable creatine. CONCLUSION: The biomarkers of HUMSCS and adipocytes were obtained and assigned. NMR spectroscopy will be a promising tool for monitoring stem cell differentiation. |
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Authors:
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Zhi-Feng Xu; Ai-Zhen Pan; Fang Yong; Chong-Yang Shen; Yao-Wen Chen; Ren-Hua Wu |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: World journal of stem cells Volume: 4 ISSN: 1948-0210 ISO Abbreviation: World J Stem Cells Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-05-11 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101535826 Medline TA: World J Stem Cells Country: China |
Other Details:
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Languages: eng Pagination: 21-7 Citation Subset: - |
Affiliation:
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Zhi-Feng Xu, Yao-Wen Chen, Ren-Hua Wu, Department of Medical Imaging, the 2nd Affiliated Hospital, Medical College of Shantou University, Shantou 515041, Guangdong Province, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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