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Human umbilical mesenchymal stem cell and its adipogenic differentiation: Profiling by nuclear magnetic resonance spectroscopy.
MedLine Citation:
PMID:  22577495     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
AIM: To study the metabolic profile of human umbilical mesenchymal stem cells (HUMSC) and adipogenic differentiation by nuclear magnetic resonance (NMR) spectroscopy.
METHODS: HUMSC isolated from human umbilical cord stroma were induced to adipocytes over 2 wk by adding dexamethasone, 3-isobutyl-1-methylxanthine, indomethacin, and insulin to the culture medium. Adipogenic differentiation was confirmed by Red O staining and transcription-polymerase chain reaction. Perchloric acid extracts of the HUMSCs and adipocytes (about 7 × 10(6)) were characterized for metabolites by using in vitro high resolution 9.4T NMR spectroscopy.
RESULTS: Several major metabolites, such as: choline, creatine, glutamate and myo-inositol, acetate, and some fatty acids/triglycerides, were observed in the MR spectroscopic pattern of HUMSCs and their adipogenic differentiation. HUMSCs are characterized by an unusually low number of NMR-detectable metabolites, high choline, acetate, glutamate and creatine content. However, the metabolic profiles of adipogenic differentiation demonstrated considerably higher methionine and fatty acids, and non-detectable creatine.
CONCLUSION: The biomarkers of HUMSCS and adipocytes were obtained and assigned. NMR spectroscopy will be a promising tool for monitoring stem cell differentiation.
Authors:
Zhi-Feng Xu; Ai-Zhen Pan; Fang Yong; Chong-Yang Shen; Yao-Wen Chen; Ren-Hua Wu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  World journal of stem cells     Volume:  4     ISSN:  1948-0210     ISO Abbreviation:  World J Stem Cells     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-05-11     Completed Date:  2012-08-23     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  101535826     Medline TA:  World J Stem Cells     Country:  China    
Other Details:
Languages:  eng     Pagination:  21-7     Citation Subset:  -    
Affiliation:
Zhi-Feng Xu, Yao-Wen Chen, Ren-Hua Wu, Department of Medical Imaging, the 2nd Affiliated Hospital, Medical College of Shantou University, Shantou 515041, Guangdong Province, China.
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