Document Detail


Human umbilical cord blood cells improve cardiac function after myocardial infarction.
MedLine Citation:
PMID:  15629157     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human umbilical cord blood (UCB) contains an abundance of immature stem/progenitor cells and has been clinically used as an alternative to bone marrow transplantation. In addition, cord blood can be obtained non-invasively, in contrast to invasive bone marrow aspiration. We investigated the potential of human UCB CD34(+) cells to improve cardiac function following myocardial infarction. Myocardial infarction was induced in Wistar rats by ligation of the left coronary artery. Either 2x10(5) human UCB CD34(+) cells or equivalent cell-free medium was injected into the injured myocardium of the rats following induction of myocardial infarction. CD34(+) cell transplantation significantly improved ventricular function as compared to the control group. Immunofluorescence staining for human CD34, CD45, and PECAM-1 revealed surviving cells in the myocardium. Our findings suggest that transplanted human cells survived and improved cardiac function following myocardial infarction. These results may show the usefulness of UCB CD34(+) cells for myocardial infarction.
Authors:
Yasutaka Hirata; Masataka Sata; Noboru Motomura; Minoko Takanashi; Yoshihiro Suematsu; Minoru Ono; Shinichi Takamoto
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  327     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-01-04     Completed Date:  2005-03-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  609-14     Citation Subset:  IM    
Affiliation:
Department of Cardiac Surgery, Graduate School of Medicine, University of Tokyo, Tokyo, Japan. h-yasu@umin.ac.jp <h-yasu@umin.ac.jp>
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD34 / metabolism
Cell Survival
Fetal Blood / cytology*,  metabolism,  transplantation*
Humans
Male
Myocardial Infarction / metabolism,  pathology*,  therapy*
Myocardium / metabolism,  pathology*
Neovascularization, Physiologic
Rats
Rats, Wistar
Stem Cell Transplantation*
Chemical
Reg. No./Substance:
0/Antigens, CD34

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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