Document Detail


Human thymocyte responsiveness to stem cell factor: synergy with interleukin-2 for the generation of NK/LAK cytotoxicity.
MedLine Citation:
PMID:  8877418     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human recombinant stem cell factor (SCF) increases the viability and cell size of a subset of thymocytes in vitro, but does not independently induce phenotypic changes on thymocytes indicative of T cell differentiation. The SCF-responsive thymocytes have characteristics of large granular cells, that do not express T, B or NK cell-related antigens, and are primarily found in immature thymocyte subsets. These large granular thymocytes do not display cytotoxic activity. However, SCF acts synergistically with IL-2 in the generation of cytotoxic effector cells from thymocyte precursors. Synergy in cytotoxicity is observed to both NK-sensitive and NK-resistant targets. Studies of the SCF receptors on thymocytes show that receptors are expressed on mature 'bright' CD3+ cells, immature 'dim' CD3+ cells as well as CD3- cells. IL-2 increases the frequency of SCF receptor-positive cells in cultured thymocytes, which may explain its synergy with SCF in the generation of NK/LAK cytotoxicity. These data show that SCF enhances the functional development of thymic NK/LAK cells in vitro.
Authors:
C H Uittenbogaart; I Schmidc; S Kiertscher; H Shau; D J Anisman; K M Zsebo; L T Clement
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Immunology letters     Volume:  52     ISSN:  0165-2478     ISO Abbreviation:  Immunol. Lett.     Publication Date:  1996 Aug 
Date Detail:
Created Date:  1997-02-06     Completed Date:  1997-02-06     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7910006     Medline TA:  Immunol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  45-52     Citation Subset:  IM    
Affiliation:
Department of Pediatrics, UCLA School of Medicine, USA.
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD / analysis
Cell Differentiation
Cells, Cultured
Child
Cytotoxicity, Immunologic*
Drug Synergism
Hematopoietic Stem Cells / drug effects*
Humans
Interleukin-2 / pharmacology*
Killer Cells, Lymphokine-Activated
Killer Cells, Natural / immunology*
Phenotype
Proto-Oncogene Proteins c-kit / analysis
Stem Cell Factor / pharmacology*
Thymus Gland / cytology*,  drug effects,  immunology
Grant Support
ID/Acronym/Agency:
CA16042/CA/NCI NIH HHS; CA42735/CA/NCI NIH HHS; HD29341/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Interleukin-2; 0/Stem Cell Factor; EC 2.7.10.1/Proto-Oncogene Proteins c-kit

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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