| Human testis in organotypic culture: application for basic or clinical research. | |
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MedLine Citation:
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PMID: 16497692 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Over recent decades, recurring efforts have been devoted to developing testicular cell or tissue cultures for basic and clinical research. However, there remains much confusion, particularly concerning the fate of human germ cells in culture. OBJECTIVE: To reassess the status of human testicular cell types as well as the ability of germ cells to divide and differentiate in organotypic culture. METHODS: Human testicular fragments were maintained for 2 weeks in culture. The viability and functionality of testicular cells were assessed using light and electronic microscopy, apoptotic cell labelling, 5-bromo-2'-deoxyuridine (BrdU) incorporation, immunohistochemistry and quantitative PCR against specific cell markers. RESULTS: A gradual loss of meiotic and post-meiotic germ cells occurred throughout the culture period, irrespective of the presence of gonadotrophins. However, all germ cell types remained traceable for up to 16 days, some still dividing and differentiating at a rate compatible with the in vivo situation. Good maintenance of the general architecture of the explants associated with clearly quantifiable levels of several somatic cell markers was observed. CONCLUSION: Although this culture model is clearly unsuitable for preparing germ cells for therapeutic purposes, it does represent a most valuable tool for testing the effects of biological and chemical agents on testicular tissue. |
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Authors:
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V Roulet; H Denis; C Staub; A Le Tortorec; B Delaleu; A P Satie; J J Patard; B Jégou; N Dejucq-Rainsford |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-02-23 |
Journal Detail:
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Title: Human reproduction (Oxford, England) Volume: 21 ISSN: 0268-1161 ISO Abbreviation: Hum. Reprod. Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-05-25 Completed Date: 2006-11-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8701199 Medline TA: Hum Reprod Country: England |
Other Details:
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Languages: eng Pagination: 1564-75 Citation Subset: IM |
Affiliation:
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INSERM, U625, GERHM, Campus de Beaulieu, Rennes, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bromodeoxyuridine
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pharmacology Cell Differentiation Cyclic AMP / metabolism DNA Fragmentation Germ Cells / metabolism Gonadotropins / metabolism Humans Immunohistochemistry In Situ Nick-End Labeling Male Meiosis Organ Culture Techniques / methods* Testis / metabolism, pathology*, ultrastructure* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Gonadotropins; 59-14-3/Bromodeoxyuridine; 60-92-4/Cyclic AMP |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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