Document Detail

Human synovial lubricin expresses sialyl Lewis x determinant and has L-selectin ligand activity.
MedLine Citation:
PMID:  22930755     Owner:  NLM     Status:  MEDLINE    
Lubricin (or proteoglycan 4 (PRG4)) is an abundant mucin-like glycoprotein in synovial fluid (SF) and a major component responsible for joint lubrication. In this study, it was shown that O-linked core 2 oligosaccharides (Galβ1-3(GlcNAcβ1-6)GalNAcα1-Thr/Ser) on lubricin isolated from rheumatoid arthritis SF contained both sulfate and fucose residues, and SF lubricin was capable of binding to recombinant L-selectin in a glycosylation-dependent manner. Using resting human polymorphonuclear granulocytes (PMN) from peripheral blood, confocal microscopy showed that lubricin coated circulating PMN and that it partly co-localized with L-selectin expressed by these cells. In agreement with this, activation-induced shedding of L-selectin also mediated decreased lubricin binding to PMN. It was also found that PMN recruited to inflamed synovial area and fluid in rheumatoid arthritis patients kept a coat of lubricin. These observations suggest that lubricin is able to bind to PMN via an L-selectin-dependent and -independent manner and may play a role in PMN-mediated inflammation.
Chunsheng Jin; Anna-Karin Hultgård Ekwall; Johan Bylund; Lena Björkman; Ruby P Estrella; John M Whitelock; Thomas Eisler; Maria Bokarewa; Niclas G Karlsson
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Publication Detail:
Type:  Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-08-28
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  287     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-22     Completed Date:  2012-12-28     Revised Date:  2013-10-22    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  35922-33     Citation Subset:  IM    
Department of Medical Biochemistry, Institute of Biomedicine, University of Gothenburg, 405 30 Gothenburg, Sweden.
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MeSH Terms
Arthritis, Rheumatoid / metabolism*,  pathology
Glycoproteins / metabolism*
Inflammation / metabolism,  pathology
L-Selectin / biosynthesis
Leukocytes, Mononuclear / metabolism*,  pathology
Middle Aged
Oligosaccharides / metabolism*
Protein Binding
Proteoglycans / metabolism*
Synovial Fluid / metabolism*
Reg. No./Substance:
0/Glycoproteins; 0/Lewis a oligosaccharide; 0/Oligosaccharides; 0/PRG4 protein, human; 0/Proteoglycans; 0/lubricin; 126880-86-2/L-Selectin

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