Document Detail


Human stomach alcohol and aldehyde dehydrogenases (ALDH): a genetic model proposed for ALDH III isozymes.
MedLine Citation:
PMID:  3214414     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Isozyme phenotypes of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) from human gastroendoscopic as well as surgical gastric biopsies were determined by starch gel electrophoresis and agarose isoelectric focusing. gamma gamma ADH isozymes were expressed predominantly in the mucosal layer of the stomach, whereas beta beta isozymes were in the muscular layer. In the 56 gastroendoscopic mucosal biopsies examined, the homozygous ADH3 1-1 phenotype was found in 75% of the samples, and the heterozygous ADH3 2-1 phenotype in 25%. Accordingly, the gene frequencies of the alleles ADH1/3 and ADH2/3 were calculated to be 0.88 and 0.12, respectively. Using a modified agarose isoelectric focusing procedure, gastric ALDH I, ALDH II, and up to five ALDH III forms could be clearly resolved. The ALDH III isozymes accounted for more than 80% of the total ALDH activities in gastric mucosa and exhibited Km values in the millimolar range for propionaldehyde at pH 9.0. Forty-five percent of the 55 gastroendoscopic biopsies studied lacked ALDH I isozyme. The complex gastric ALDH III isozyme phenotypes seen in these biopsies fall into three patterns. They can be interpreted by a genetic hypothesis, based on a dimeric molecule, in which there are two separate genes, ALDH3a and ALDH3b, with the ALDH3b locus exhibiting polymorphism. The homozygous phenotypes ALDH3b 1-1 and ALDH3b 2-2 were found to be 4 and 76%, respectively, and the heterozygous ALDH3b 2-1 phenotype 20%, of the total. Therefore, the allele frequencies for ALDH1/3b and ALDH2/3b were calculated to be 0.14 and 0.86, respectively. Several lines of biochemical evidence consistent with this genetic model are discussed.
Authors:
S J Yin; T C Cheng; C P Chang; Y J Chen; Y C Chao; H S Tang; T M Chang; C W Wu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical genetics     Volume:  26     ISSN:  0006-2928     ISO Abbreviation:  Biochem. Genet.     Publication Date:  1988 Jun 
Date Detail:
Created Date:  1989-02-23     Completed Date:  1989-02-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0126611     Medline TA:  Biochem Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  343-60     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, National Defense Medical Center, Taipei, Taiwan, Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Alcohol Dehydrogenase / genetics*,  metabolism
Aldehyde Dehydrogenase / genetics*,  metabolism
Continental Population Groups
Electrophoresis, Agar Gel
Electrophoresis, Starch Gel
Ethnic Groups
Gastric Mucosa / enzymology*
Humans
Isoenzymes / genetics*,  metabolism
Models, Genetic*
Stomach / enzymology*
Chemical
Reg. No./Substance:
0/Isoenzymes; EC 1.1.1.1/Alcohol Dehydrogenase; EC 1.2.1.3/Aldehyde Dehydrogenase

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