Document Detail


Human squamous cell carcinoma. Establishment and characterization of new permanent cell lines.
MedLine Citation:
PMID:  7295166     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Squamous cell carcinoma is the most common of human cancers, and yet because it is poorly represented by cultured cell lines, little is known about the characteristic cell biology and the cell-surface antigenic phenotypes of such tumors. To develop a continuously available source of squamous cell carcinoma for repeated and reproducible serologic analysis and for better understanding of its biologic characteristics, tissue culture methods and nude mice were used to establish new cell lines of squamous carcinoma. Special media, serum supplements from several sources, and methods of handling fresh tissue specimens were all examined as a means of improving the survival of tumor cell lines. Several new cell lines were established. Features characteristic of a squamous cell origin, eg, microvilli, desmosomes, tonofilaments, and the squamous cell differentiation antigen (pemphigus antigen), were found. The clinical course of disease in individual donor patients has been examined.
Authors:
C J Krause; T E Carey; R W Ott; C Hurbis; K D McClatchey; J A Regezi
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Publication Detail:
Type:  Case Reports; In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Archives of otolaryngology (Chicago, Ill. : 1960)     Volume:  107     ISSN:  0003-9977     ISO Abbreviation:  Arch Otolaryngol     Publication Date:  1981 Nov 
Date Detail:
Created Date:  1981-12-21     Completed Date:  1981-12-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376526     Medline TA:  Arch Otolaryngol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  703-10     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Aged
Animals
Antigens, Neoplasm / immunology
Carcinoma, Squamous Cell / immunology,  pathology*
Cell Line*
Humans
Male
Mice
Mice, Nude
Mouth Neoplasms / immunology,  pathology*
Neoplasm Transplantation
Neoplasms, Experimental
Grant Support
ID/Acronym/Agency:
CA-00621/CA/NCI NIH HHS; CA-28564/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, Neoplasm

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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