| Human spermatozoa vitrified in the absence of permeable cryoprotectants: birth of two healthy babies. | |
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MedLine Citation:
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PMID: 22281078 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Herein, we report the birth of two healthy babies to a woman following intracytoplasmic sperm injection (ICSI) using motile spermatozoa vitrified without permeable cryoprotectants. Spermatozoa (in a case of oligoasthenoteratozoospermia) were cooled in cut standard straws in human tubal fluid supplemented with 0.5% human serum albumin and 0.25M sucrose. Sperm motility, capacitation-like changes, acrosome reaction and mitochondrial membrane potential (MMP) were compared in fresh and vitrified spermatozoa. Eight mature (MII) oocytes were microinjected with the vitrified-warmed motile spermatozoa. Although the motility of vitrified-warmed spermatozoa was markedly lower than that of fresh spermatozoa (60% v. 90%, respectively), there were no immediate visible differences in the percentages of capacitated and acrosome-reacted vitrified and fresh spermatozoa (10% v. 8% and 5% v. 8%, respectively). However, the MMP in vitrified spermatozoa was apparently adversely affected in the ejaculate used for ICSI compared with fresh spermatozoa (63% v. 96% spermatozoa with high MMP). Eighteen hours later, six oocytes showed signs of normal fertilisation. Two-pronuclear oocytes were cultured in vitro for 24h and two four-blastomere embryos were transferred. Two healthy girls were born at term. Our findings suggest that permeable cryoprotectant-free vitrification can be applied successfully for some procedures in assisted reproduction, in particular in ICSI with motile vitrified spermatozoa, to achieve normal pregnancy and birth. |
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Authors:
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Vladimir Isachenko; Evgenia Isachenko; Anna M Petrunkina; Raul Sanchez |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Reproduction, fertility, and development Volume: 24 ISSN: 1031-3613 ISO Abbreviation: Reprod. Fertil. Dev. Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-01-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8907465 Medline TA: Reprod Fertil Dev Country: Australia |
Other Details:
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Languages: eng Pagination: 323-6 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynaecology, University Women Hospital, Kerpener Str. 34, 50931 Cologne, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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