Document Detail

Human sperm motility--enhancement and inhibition measured by laser Doppler spectroscopy.
MedLine Citation:
PMID:  6305236     Owner:  NLM     Status:  MEDLINE    
Laser Doppler spectroscopy is an excellent instrument to measure motility parameters of human spermatozoa. Experiments have been performed by the aid of a now commercially available instrumental setup called LAZYMOT to study enhancement and inhibition as well as influences of different physical treatments on the motility of human spermatozoa. The motility enhancing influence of Kallikrein (up to 40% with 4 KU/ml) and prostaglandin E2 could be proven, thereby verifying some sort of mechanism of action within the Kallikrein-Kinin system. Furthermore tested substances in this context were bradykinin (no effect), caffeine (enhancement), salicylic acid (slight decrease), and other prostaglandins (no effect). The influence of washing, centrifugation, resuspension liquid, and temperature has been examined. It turned out that the lowest motility degradation is to be achieved by washing twice at a pH value of 7.4 at forces of 200 g for 10 min each time. The optimal temperature during this process and the measurements thereafter was found to be 36 degrees C. Unsaturated fatty acids like arachidonic acid have been found to be very strong motility inhibiting substances, which can totally block any movement of spermatozoa.
R Hartmann; R Steiner; N Hofmann; R Kaufmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Andrologia     Volume:  15     ISSN:  0303-4569     ISO Abbreviation:  Andrologia     Publication Date:    1983 Mar-Apr
Date Detail:
Created Date:  1983-07-08     Completed Date:  1983-07-08     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0423506     Medline TA:  Andrologia     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  120-34     Citation Subset:  IM    
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MeSH Terms
Bucladesine / pharmacology
Caffeine / pharmacology
Cyclic AMP / pharmacology
Doppler Effect*
Hydrogen-Ion Concentration
Kallikreins / pharmacology
Physical Phenomena
Prostaglandins E / pharmacology
Spectrum Analysis / methods*
Sperm Motility / drug effects*
Reg. No./Substance:
0/Prostaglandins E; 362-74-3/Bucladesine; 363-24-6/Dinoprostone; 58-08-2/Caffeine; 60-92-4/Cyclic AMP; EC 3.4.21.-/Kallikreins

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