Document Detail


Human skeletal muscle glycogen utilization in exhaustive exercise: role of subcellular localization and fibre type.
MedLine Citation:
PMID:  21486810     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although glycogen is known to be heterogeneously distributed within skeletal muscle cells, there is presently little information available about the role of fibre types, utilization and resynthesis during and after exercise with respect to glycogen localization. Here, we tested the hypothesis that utilization of glycogen with different subcellular localizations during exhaustive arm and leg exercise differs and examined the influence of fibre type and carbohydrate availability on its subsequent resynthesis. When 10 elite endurance athletes (22 ± 1 years, VO2 max = 68 ± 5 ml kg-1 min-1, mean ± SD) performed one hour of exhaustive arm and leg exercise, transmission electron microscopy revealed more pronounced depletion of intramyofibrillar than of intermyofibrillar and subsarcolemmal glycogen. This phenomenon was the same for type I and II fibres, although at rest prior to exercise, the former contained more intramyofibrillar and subsarcolemmal glycogen than the latter. In highly glycogen-depleted fibres, the remaining small intermyofibrillar and subsarcolemmal glycogen particles were often found to cluster in groupings. In the recovery period, when the athletes received either a carbohydrate-rich meal or only water the impaired resynthesis of glycogen with water alone was associated primarily with intramyofibrillar glycogen. In conclusion, after prolonged high-intensity exercise the depletion of glycogen is dependent on subcellular localization. In addition, the localization of glycogen appears to be influenced by fibre type prior to exercise, as well as carbohydrate availability during the subsequent period of recovery. These findings provide insight into the significance of fibre type-specific compartmentalization of glycogen metabolism in skeletal muscle during exercise and subsequent recovery. .
Authors:
Joachim Nielsen; Hans-Christer Holmberg; Henrik D Schrøder; Bengt Saltin; Niels Ortenblad
Publication Detail:
Type:  Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't     Date:  2011-04-04
Journal Detail:
Title:  The Journal of physiology     Volume:  589     ISSN:  1469-7793     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-06-02     Completed Date:  2011-10-06     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2871-85     Citation Subset:  IM    
Affiliation:
Institute of Sports Science and Clinical Biomechanics, University of Southern Denmark, Odense, Denmark, DK-5230 Odense M, Denmark. jnielsen@health.sdu.dk
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Arm
Dietary Carbohydrates / metabolism
Exercise / physiology*
Glycogen / metabolism*,  ultrastructure
Humans
Intracellular Space / metabolism*
Male
Microscopy, Electron, Transmission
Muscle Fibers, Fast-Twitch / metabolism
Muscle Fibers, Skeletal / cytology*,  metabolism*,  ultrastructure
Muscle Fibers, Slow-Twitch / metabolism,  ultrastructure
Muscle, Skeletal / cytology,  metabolism*
Quadriceps Muscle / metabolism
Skiing / physiology*
Young Adult
Chemical
Reg. No./Substance:
0/Dietary Carbohydrates; 9005-79-2/Glycogen
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Coupling of the phosphatase activity of Ci-VSP to its voltage sensor activity over the entire range ...
Next Document:  In vivo two-photon uncaging of glutamate revealing the structure-function relationships of dendritic...