Document Detail

Human serum protein enhances HIV-1 replication and up-regulates the transcription factor AP-1.
MedLine Citation:
PMID:  23047699     Owner:  NLM     Status:  MEDLINE    
In vitro studies on HIV (HIV-1) replication and neutralization are usually performed in human cell cultures supplemented with FBS instead of human serum (HS). Here we show that in contrast to FBS, addition of increasing amounts of human serum from noninfected donors to the cell culture directly correlates with an increase in HIV-1 replication in vitro. This effect is independent of cell line, virus strain, or batch of pooled human serum used. We found that human serum affects viral transcription in a dose-dependent manner by activating the activator protein-1 (AP-1) member proteins c-FOS, JunD, and JunB in TZM-bl cells. Analysis of the human serum component responsible for this effect indicates that it is a protein having a molecular mass between 250 and 300 kDa. This serum protein, HIV-1 enhancing serum protein (HESP), might promote viral transcription in vivo and consequently play a role in disease progression.
Maria F Perdomo; Waltteri Hosia; Alenka Jejcic; Garry L Corthals; Anders Vahlne
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-10-09
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  109     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-24     Completed Date:  2013-01-07     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  17639-44     Citation Subset:  IM    
Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge F68, SE-14186 Huddinge, Sweden.
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MeSH Terms
Blood Proteins / physiology*
HIV-1 / physiology*
Transcription Factor AP-1 / physiology*
Transcription, Genetic
Up-Regulation / physiology*
Virus Replication / physiology*
Reg. No./Substance:
0/Blood Proteins; 0/Transcription Factor AP-1

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