Document Detail

Human serum albumin improves arterial dysfunction during early resuscitation in mouse endotoxic model via reduced oxidative and nitrosative stresses.
MedLine Citation:
PMID:  17991713     Owner:  NLM     Status:  MEDLINE    
Human serum albumin (HSA) is used as a resuscitation fluid in sepsis. This study investigated the potential protective properties of HSA on vascular function in a mouse endotoxic model in terms of oxidative and nitrosative stresses. Swiss mice were treated with either lipopolysaccharide (LPS) (50 mg/kg i.p.) or vehicle. One and five hours later, mice were infused with HSA (4%, 10 ml/kg), normal saline (0.9% NaCl, 30 ml/kg), or no fluid. Six hours after treatment, vascular reactivity was assessed on aortae and small mesenteric arteries. Measurements of NO and superoxide anion (O2(-)) by spin trapping and nuclear factor (NF)-kappaB, inducible NO synthase (iNOS), and peroxynitrite by Western blotting and immunohistochemical studies were conducted. HSA partially prevented the reduction of blood pressure induced by LPS and completely prevented both vascular hyporeactivity to phenylephrine and myogenic tone as well as endothelial dysfunction induced by the endotoxin. This was associated with a decreased up-regulation of NF-kappa B, iNOS, and peroxynitrite in the vascular wall. LPS-induced tissue increases in both NO and O2(-) production was decreased by HSA. These data demonstrate the protective effect of HSA treatment in experimental endotoxic shock by reducing the inflammatory process leading to oxidative and nitrosative stresses and vascular hyporeactivity.
Ferhat Meziani; Hélène Kremer; Angela Tesse; Céline Baron-Menguy; Cyrille Mathien; H Ahmed Mostefai; Nunzia Carusio; Francis Schneider; Pierre Asfar; Ramaroson Andriantsitohaina
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-11-08
Journal Detail:
Title:  The American journal of pathology     Volume:  171     ISSN:  0002-9440     ISO Abbreviation:  Am. J. Pathol.     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-12-10     Completed Date:  2008-02-08     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370502     Medline TA:  Am J Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1753-61     Citation Subset:  AIM; IM    
INSERM UMR 771 Angers, CNRS UMR 6214, Faculté de Médecine, rue Haute de Reculée, 49000 Angers, France.
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MeSH Terms
Aorta / drug effects
Blood Pressure / drug effects*
Disease Models, Animal
Heart Rate / drug effects
Lipopolysaccharides / toxicity
Mesenteric Arteries / drug effects
NF-kappa B / analysis
Nitric Oxide / analysis
Nitric Oxide Synthase Type II / analysis
Oxidative Stress / drug effects
Serum Albumin / pharmacology,  therapeutic use*
Shock, Septic / drug therapy*
Superoxides / analysis
Vasodilation / drug effects*
Reg. No./Substance:
0/Lipopolysaccharides; 0/NF-kappa B; 0/Serum Albumin; 10102-43-9/Nitric Oxide; 11062-77-4/Superoxides; EC Oxide Synthase Type II

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