Document Detail


Human serum albumin cysteinylation is increased in end stage renal disease patients and reduced by hemodialysis: mass spectrometry studies.
MedLine Citation:
PMID:  23215783     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Abstract - The aim of the present work was to monitor the covalent modifications of human serum albumin (HSA) in end stage renal diseases (ESRD) non diabetic patients, before and after hemodialysis (HD), by direct infusion electrospray mass spectrometry (ESI-MS). Human serum samples were collected from healthy subjects (n = 10, 20-60 yr) and age-matched ESRD patients (n = 8) before and after HD, purified by affinity chromatography and analyzed by a triple-quadrupole mass spectrometer. The deconvoluted spectra from healthy subjects were all characterized by three peaks attributed to non-glycated mercaptoalbumin (HSA-SH) and to the corresponding adducts with cysteine (HSA-Cys) and glucose (HSA-Glc); relative contents: mercaptoalbumin in both glycated and non-glycated form, HSA-SHt (74 ± 6%), HSA-Cys (26 ± 5 %) and HSA-Glc (24 ± 3 %). HSA isolated from ESRD patients before HD was characterized by a significant reduction of HSA-SHt (42 ± 7%), and by a concomitant increase of the HSA-Cys adduct (58 ± 7%). Hemodialysis significantly reduced the cysteinylated form (37 ± 7%) and restored HSA-SHt (63 ± 8%) in all the ESRD patients. The mechanism of thiol oxidation and cysteinylation was then studied by mass spectrometry, using LQQCPF as a model peptide and H(2)O(2) as an oxidizing agent. Abbreviations: Albumin (HSA); non-glycated mercaptoalbumin (HSA-SH); glycated mercaptoalbumin (HSA-Glc); cysteinylated albumin (HSA-Cys); sum of glycated and non-glycated mercaptoalbumin (HSA-SHt); end stage renal diseases (ESRD); hemodialysis (HD); reactive carbonyl species (RCS); 4-hydroxy-trans-2-nonenal (HNE).
Authors:
Luca Regazzoni; Lucia Del Vecchio; Alessandra Altomare; Kyung-Jin Yeum; Daniele Cusi; Francesco Locatelli; Marina Carini; Giancarlo Aldini
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-6
Journal Detail:
Title:  Free radical research     Volume:  -     ISSN:  1029-2470     ISO Abbreviation:  Free Radic. Res.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9423872     Medline TA:  Free Radic Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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