| Human seminal vesicle-specific antigen is a substrate for prostate-specific antigen (or P-30). | |
| | |
MedLine Citation:
|
PMID: 2460148 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Human seminal vesicle and prostatic fluids were obtained separately and reconstituted in vitro to test the hypothesis that proteolytic enzymes of prostatic origin would degrade seminal vesicle-specific antigen (SVSA). Using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblot analysis to follow the fate of SVSA over time, we found that upon mixing the two secretions, SVSA was converted to forms of intermediate and low molecular weight identical to transformations seen in normal liquefied ejaculates. Diisoproprylfluorophophate, a serine protease inhibitor, prevented this degradation, indicating serine protease involvement in the proteolysis of SVSA. Prostate-specific antigen (PSA; also known as P-30), recently identified as a serine protease, was examined for its ability to mimic the effects of prostatic fluid on SVSA. Purified PSA catalyzed degradation of SVSA to produce proteolytic fragments that comigrated and were immunologically related to SVSA fragments produced by prostatic fluid. Purified PSA in the presence of serine protease inhibitors was unable to degrade SVSA. These results demonstrate that SVSA is a substrate for PSA during human semen liquefaction. |
| | |
Authors:
|
R S McGee; J C Herr |
Related Documents
:
|
1725158 - Metastatic prostatic adenocarcinoma-diagnosed by bronchoalveolar lavage and tumour mark... 10197848 - Kinetics of postbiopsy levels of serum free prostate-specific antigen and percent free ... 18549428 - Highly specific detection of prostate-specific antigen-positive cells in the blood of p... 16192588 - Postoperative nomogram predicting the 10-year probability of prostate cancer recurrence... 19487288 - Expression of the k303r estrogen receptor-alpha breast cancer mutation induces resistan... 3409168 - Plasma tetranectin is reduced in cancer and related to metastasia. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Biology of reproduction Volume: 39 ISSN: 0006-3363 ISO Abbreviation: Biol. Reprod. Publication Date: 1988 Sep |
Date Detail:
|
Created Date: 1988-12-12 Completed Date: 1988-12-12 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 499-510 Citation Subset: IM |
Affiliation:
|
Department of Anatomy and Cell Biology, University of Virginia Medical Center, Charlottesville 22908. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antigens, Neoplasm
/
immunology* Blotting, Western Body Fluids / analysis Electrophoresis, Polyacrylamide Gel Humans Isoflurophate / pharmacology Male Molecular Weight Peptide Hydrolases / metabolism Phenylmethylsulfonyl Fluoride / pharmacology Prostate-Specific Antigen Semen / analysis Seminal Vesicles / immunology* |
| Grant Support | |
ID/Acronym/Agency:
|
HD 16767/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, Neoplasm; 329-98-6/Phenylmethylsulfonyl Fluoride; 55-91-4/Isoflurophate; EC 3.4.-/Peptide Hydrolases; EC 3.4.21.77/Prostate-Specific Antigen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: In vitro effects of pregnancy-associated plasma protein-A: artifacts due to heparin.
Next Document: Chemotactic activity of recombinant human granulocyte colony-stimulating factor.