| Human saliva cortisone and cortisol simultaneous analysis using reverse phase HPLC technique. | |
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MedLine Citation:
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PMID: 19393639 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Hyper-hypo tension (like Cushing's syndrome, apparent mineralocorticoid excess syndrome and Addison's disease) diagnostic laboratory requires cortisol (F) analysis. The simultaneous analysis of human saliva F and cortisone (E), the inactive F metabolite, by solid phase extraction and RP-HPLC was studied. METHODS: Saliva/standard samples were C18-SPE extracted, dried and resuspended. E and F were analysed by isocratic RP-HPLC (acetonitrile/water 27/73%) and UV detection. In the morning and in the evening Salivette stimulated saliva specimens were collected from healthy volunteers. RESULTS: The E and F calibration curve ranges were 11.0-110.0 and 5.5-55.0 nmol/l respectively. The LOD was 0.2 and 0.1 nmol/l for E and F respectively. The intra and inter assay CVs were respectively 2.7-6.6 and 5.6-7.0% for E and 5.8-7.0 and 11.7-13.1% for F. The E and F spiked saliva sample recovery was 99% and 88% respectively. Saliva specimen stability was validated. E and F saliva levels in healthy volunteers were significantly (p<0.001) higher at 8 a.m. compared with 11 p.m. (26.4+/-8.9 vs. 4.3+/-2.9 nmol/l for E; 11.1+/-4.0 vs. 2.5+/-1.5 nmol/l for F, respectively). CONCLUSIONS: This method is suitable for periodic analyses in a clinical biochemistry laboratory for endocrinology investigation purposes, simultaneously analysing E and F levels in a saliva specimen. |
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Authors:
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Elio F De Palo; Giorgia Antonelli; Arianna Benetazzo; Maddalena Prearo; Rosalba Gatti |
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Publication Detail:
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Type: Journal Article Date: 2009-04-22 |
Journal Detail:
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Title: Clinica chimica acta; international journal of clinical chemistry Volume: 405 ISSN: 1873-3492 ISO Abbreviation: Clin. Chim. Acta Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-06-02 Completed Date: 2009-07-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1302422 Medline TA: Clin Chim Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 60-5 Citation Subset: IM |
Affiliation:
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Section of Clinical Biochemistry, Department of Medical Diagnostic Sciences and Special Therapies, University of Padua, 35100 Padua, Italy. eliof.depalo@unipd.it |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Chromatography, High Pressure Liquid / methods* Cortisone / analysis* Female Humans Hydrocortisone / analysis* Hydrophobicity* Indicator Dilution Techniques Male Saliva / chemistry* Solid Phase Extraction |
| Chemical | |
Reg. No./Substance:
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50-23-7/Hydrocortisone; 53-06-5/Cortisone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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