| Human retinal pigment epithelium-induced CD4+CD25+ regulatory T cells suppress activation of intraocular effector T cells. | |
| | |
MedLine Citation:
|
PMID: 20350837 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Murine retinal pigment epithelial (RPE) cells suppress T-cell activation by releasing soluble inhibitory factors and promote the generation of regulatory T cells in vitro. These T cells exposed to RPE supernatants (RPE-induced Treg cells) can suppress the activation of bystander effector T cells via the production of transforming growth factor-beta (TGFbeta). In the present study, we showed that human RPE-induced Treg cells are also able to acquire regulatory function when human RPE cell lines were pretreated with recombinant TGF beta 2. These RPE-induced Treg cells produced TGF beta 1 and IL-10 but not IFN gamma, and they significantly suppressed the activation of target cell lines and intraocular T-cell clones established from patients with active uveitis. Moreover, CD4(+)CD25(+) RPE-induced Treg cells expressed CTLA-4 and Foxp3 molecules, and the CD25(+) Treg cells profoundly suppressed the T-cell activation. Thus, in vitro manipulated Treg cells acquire functions that participate in the establishment of immune tolerance in the eye. |
| | |
Authors:
|
Shintaro Horie; Sunao Sugita; Yuri Futagami; Yukiko Yamada; Manabu Mochizuki |
Related Documents
:
|
16903917 - Regulatory t-cell physiology and application to treat autoimmunity. 19348917 - Maternal farm exposure modulates neonatal immune mechanisms through regulatory t cells. 17712427 - Low numbers of foxp3 positive regulatory t cells are present in all developmental stage... 14742677 - Barbiturates directly inhibit the calmodulin/calcineurin complex: a novel mechanism of ... 16903917 - Regulatory t-cell physiology and application to treat autoimmunity. 17272507 - Distinctive nk-cell receptor repertoires sustain high-level constitutive nk-cell activa... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-29 |
Journal Detail:
|
Title: Clinical immunology (Orlando, Fla.) Volume: 136 ISSN: 1521-7035 ISO Abbreviation: Clin. Immunol. Publication Date: 2010 Jul |
Date Detail:
|
Created Date: 2010-06-23 Completed Date: 2010-08-06 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100883537 Medline TA: Clin Immunol Country: United States |
Other Details:
|
Languages: eng Pagination: 83-95 Citation Subset: IM |
Copyright Information:
|
(c) 2010 Elsevier Inc. All rights reserved. |
Affiliation:
|
Department of Ophthalmology & Visual Science, Tokyo Medical and Dental University, Graduate School of Medical and Dental Sciences, Tokyo, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antibodies, Monoclonal
/
immunology,
pharmacology CD4-Positive T-Lymphocytes / immunology, metabolism Cell Line Cell Proliferation Culture Media, Conditioned / pharmacology Dinoprostone / metabolism Epithelial Cells / drug effects, immunology, metabolism Eye / cytology, immunology* Forkhead Transcription Factors / metabolism Gene Expression / drug effects, genetics Humans Immune Tolerance / immunology* Interferon-gamma / metabolism Interleukin-10 / metabolism Interleukin-2 Receptor alpha Subunit / metabolism* Lymphocyte Activation / immunology RNA, Small Interfering / genetics Receptors, Transforming Growth Factor beta / genetics Retinal Pigment Epithelium / cytology, immunology* T-Lymphocytes / immunology*, metabolism T-Lymphocytes, Regulatory / drug effects, immunology*, metabolism Th1 Cells / immunology, metabolism Thrombospondin 1 / metabolism Transforming Growth Factor beta / antagonists & inhibitors, immunology Transforming Growth Factor beta1 / genetics, metabolism Transforming Growth Factor beta2 / genetics, metabolism, pharmacology Uveitis / immunology |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies, Monoclonal; 0/Culture Media, Conditioned; 0/FOXP3 protein, human; 0/Forkhead Transcription Factors; 0/IL10 protein, human; 0/IL2RA protein, human; 0/Interleukin-2 Receptor alpha Subunit; 0/RNA, Small Interfering; 0/Receptors, Transforming Growth Factor beta; 0/Thrombospondin 1; 0/Transforming Growth Factor beta; 0/Transforming Growth Factor beta1; 0/Transforming Growth Factor beta2; 130068-27-8/Interleukin-10; 363-24-6/Dinoprostone; 82115-62-6/Interferon-gamma |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Slow (1 Hz) repetitive transcranial magnetic stimulation (rTMS) induces a sustained change in cortic...
Next Document: Late termination of pregnancy and foetal reduction for foetal anomaly.