Document Detail

Human recombinant IL-4 induces activated B lymphocytes to produce IgG and IgM.
MedLine Citation:
PMID:  2971718     Owner:  NLM     Status:  MEDLINE    
In this report, we describe a novel biologic activity of IL-4 namely, its ability to induce activated human B cells to produce IgM. Staphylococcus aureus Cowan I-activated blasts prepared from high density tonsil B cells were found to secrete IgG and IgM, but no IgE, when cultured in the presence of rIL-4. The differentiating activity of rIL-4 was totally blocked by a neutralizing anti-IL-4 antiserum, therefore demonstrating that the IgG/IgM-inducing activity of rIL-4 was an intrinsic property of IL-4. rIL-4 was only minimally inducing Ig production of blasts prepared from low density B cells, whereas it induced B cell blasts prepared from high density B cells to secrete a high amount of Ig. Delayed additions of the neutralizing anti-IL-4 antiserum demonstrated that a 48-h contact between IL-4 and B cell blasts was required for optimal Ig production. The IL-4-mediated IgG and IgM production was neither suppressed by IFN-gamma nor by anti-CD23 mAb 25, whereas these agents have been shown earlier to inhibit IgE production of enriched B cells cultured in the presence of IL-4. These data indicate that the IgG/IgM-inducing activity of IL-4 is not regulated like the IL-4-induced IgE production by enriched B cells.
T Defrance; B Vanbervliet; J Pène; J Banchereau
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  141     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1988 Sep 
Date Detail:
Created Date:  1988-11-03     Completed Date:  1988-11-03     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2000-5     Citation Subset:  AIM; IM    
UNICET, Laboratory for Immunological Research, Dardilly, France.
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MeSH Terms
Antibodies, Monoclonal / physiology
Antigens, Differentiation, B-Lymphocyte / immunology
B-Lymphocytes / drug effects*,  immunology,  metabolism
Binding, Competitive
Immune Sera / pharmacology
Immunoglobulin G / biosynthesis*
Immunoglobulin M / biosynthesis*
Interferon-gamma / pharmacology
Interleukins / immunology,  pharmacology*
Lymphocyte Activation / drug effects*
Neutralization Tests
Receptors, Fc / immunology
Receptors, IgE
Recombinant Proteins / pharmacology
Staphylococcal Vaccines
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Differentiation, B-Lymphocyte; 0/Immune Sera; 0/Immunoglobulin G; 0/Immunoglobulin M; 0/Interleukins; 0/Receptors, Fc; 0/Receptors, IgE; 0/Recombinant Proteins; 0/Staphylococcal Vaccines; 207137-56-2/Interleukin-4; 82115-62-6/Interferon-gamma

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