| Human prostatic acid phosphatase directly stimulates collagen synthesis and alkaline phosphatase content of isolated bone cells. | |
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MedLine Citation:
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PMID: 1653783 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human prostatic acid phosphatase (hPAP) directly enhances the differentiated characteristics of isolated bone cells in vitro. This enzyme, when added to cell cultures for 24 h in vitro stimulates collagen synthesis and the production of alkaline phosphatase. The effects are dose dependent, with statistically significant effects occurring from 0.1-100 nM hPAP. Concentrations higher than 100 nM do not evoke greater effects. The maximal effect of hPAP occurs between 12 and 24 h of exposure. The cells stimulated to the greatest degree are osteoprogenitor cells and osteoblasts. Fibroblasts isolated from the same tissue show a lesser sensitivity to hPAP. hPAP has no detectable effect on cell proliferation, as measured by radiolabeled thymidine incorporation or total DNA synthesis. None of the observations reported in this work can be attributed to contaminating proteins in the hPAP preparation. hPAP was radiolabeled with 125I and was used for affinity binding and cross-linking studies. Scatchard analysis of specific binding indicated the presence of 1.0 X 10(5) high affinity binding sites/cell, with a Kd of 6.5 nM. Cross-linking studies demonstrated the presence of one 320-kDa binding complex. The pH profile and kinetic determinations of Km and maximum velocity for hPAP were similar to those previously reported, except for the finding of positive cooperativity of the substrate with the enzyme under the conditions of our assay. We believe that the direct stimulation of bone-forming cells by hPAP may contribute to the sclerotic nature of skeletal bone around sites of neoplastic prostatic metastases and that the effect of the enzyme is probably mediated by a plasma membrane receptor. |
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Authors:
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M Ishibe; R N Rosier; J E Puzas |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of clinical endocrinology and metabolism Volume: 73 ISSN: 0021-972X ISO Abbreviation: J. Clin. Endocrinol. Metab. Publication Date: 1991 Oct |
Date Detail:
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Created Date: 1991-10-17 Completed Date: 1991-10-17 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0375362 Medline TA: J Clin Endocrinol Metab Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 785-92 Citation Subset: AIM; IM |
Affiliation:
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Department of Orthopaedics, University of Rochester, New York 14642. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acid Phosphatase
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pharmacology* Alkaline Phosphatase / analysis*, metabolism Animals Bone and Bones / cytology*, enzymology, metabolism Cell Separation Cells, Cultured Collagen / metabolism* Dose-Response Relationship, Drug Fibroblasts / metabolism Hydrogen-Ion Concentration Insulin-Like Growth Factor II / metabolism Iodine Radioisotopes / diagnostic use Male Prostate / enzymology* Rats Receptors, Cell Surface / metabolism, physiology |
| Grant Support | |
ID/Acronym/Agency:
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AR-28420/AR/NIAMS NIH HHS; AR-38618/AR/NIAMS NIH HHS; AR-38945/AR/NIAMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Iodine Radioisotopes; 0/Receptors, Cell Surface; 67763-97-7/Insulin-Like Growth Factor II; 9007-34-5/Collagen; EC 3.1.3.1/Alkaline Phosphatase; EC 3.1.3.2/Acid Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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