| Human proinsulin, VIII: studies on the S-tritylation of reduced proinsulin, insulin A and B chains and their detritylation. | |
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MedLine Citation:
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PMID: 6995254 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Reduced proinsulin and insulin A and B chains were selectively and quantitatively converted to hexa(S-trityl)proinsulin, tetra(S-trityl)A chain and di(S-trityl)B chain. These derivatives were used as models to investigate the quantitative removal of S-trityl groups. Amongst the various detritylation methods studied, the best procedure was found to be acidolytic cleavage with trifluoroacetic acid in the presence of thiophenol or benzylmercaptan as cation scavengers. The detritylated derivatives, upon oxidative sulphitolysis, yielded electrophoretically homogeneous hexa(S-sulphonate)proinsulin, tetra(S-sulphonate)A chain and di(S-sulphonate)B chain. The hexa(S-sulphonate)proinsulin was reduced and reoxidized to proinsulin. |
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Authors:
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E E Büllesbach; W Danho; H J Helbig; H Zahn |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Hoppe-Seyler's Zeitschrift für physiologische Chemie Volume: 361 ISSN: 0018-4888 ISO Abbreviation: Hoppe-Seyler's Z. Physiol. Chem. Publication Date: 1980 |
Date Detail:
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Created Date: 1980-10-24 Completed Date: 1980-10-24 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 2985060R Medline TA: Hoppe Seylers Z Physiol Chem Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 865-73 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Chemical Phenomena Chemistry Insulin / metabolism* Oxidation-Reduction Proinsulin / metabolism* |
| Chemical | |
Reg. No./Substance:
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11061-68-0/Insulin; 9035-68-1/Proinsulin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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