Document Detail


Human plasma triglyceride labeling after high sucrose feeding. II. Study on triglyceride kinetics and postheparin lipolytic activity.
MedLine Citation:
PMID:  165366     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Kinetic studies of the very-low-density lipoprotein triglycerides (VLDL-TG) turnover by endogenous labeling with glycerol-2-3-H were performed in 13 patients in the postabsorptive state, first after 10-14 days on a low-sucrose high-starch diet, then again after 10-14 days of isocaloric high-sucrose low-starch diet (HSD). After HSD, a significant decrease in the fractional turnover rates of VLDL-TG was observed, as well as a modest but significant increase in its pool size, but the net turnover rates remained unchanged. Using Michaelis-Menten formulation, we have further calculated the Vmax and Km's of the removal system for VLDL-TG and found that the Vmax and Km's do not differ significantly between the two dietary periods. These results suggest that the removal mechanism for VLDL-TG has not changed after 10-14 days on the HSD, at least when the patients are studied in the postabsorptive state. Measurements of postheparin lipolytic acitivty under fed condition in 17 patients (including the 13 patients above) have shown a decrease after HSD. However, a defect in the removal of plasma-TG related to decreased activity of tissue-lipoprotein lipase in the fed state has not been conclusively uncovered by the kinetic studies performed in the postabsorptive state, and cannot contribute significantly to the expansion of VLDL-TG pool.
Authors:
C H Wu; W W Shreeve
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Metabolism: clinical and experimental     Volume:  24     ISSN:  0026-0495     ISO Abbreviation:  Metab. Clin. Exp.     Publication Date:  1975 Jun 
Date Detail:
Created Date:  1975-08-07     Completed Date:  1975-08-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375267     Medline TA:  Metabolism     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  755-66     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Calorimetry
Cholesterol / blood
Dietary Carbohydrates*
Fasting
Female
Heparin / pharmacology*
Humans
Kinetics
Lipid Mobilization / drug effects*
Lipoprotein Lipase / metabolism
Lipoproteins, VLDL / blood*
Male
Middle Aged
Obesity / blood
Starch / pharmacology
Sucrose / pharmacology*
Time Factors
Triglycerides / blood*
Chemical
Reg. No./Substance:
0/Dietary Carbohydrates; 0/Lipoproteins, VLDL; 0/Triglycerides; 57-50-1/Sucrose; 57-88-5/Cholesterol; 9005-25-8/Starch; 9005-49-6/Heparin; EC 3.1.1.34/Lipoprotein Lipase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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