| Human placental apurinic/apyrimidinic endonuclease. Its isolation and characterization. | |
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MedLine Citation:
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PMID: 7142159 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An endonuclease from human placenta has been purified to apparent homogeneity, which acts specifically on DNA containing either apurinic or apyrimidinic sites. The isolation procedure, which results in a 20,000-fold purification and an overall yield of 15%, employs chromatography on a gel of octyl succinic anhydride coupled to agarose by diaminohexane spacers, isoelectric focusing, Sephadex G-75 chromatography, and DNA agarose affinity chromatography. Under conditions in which proteolysis is minimized, this enzyme appears to be the major species of apurinic/apyrimidinic endonuclease. The endonuclease is a monomeric protein with an apparent Mr = 37,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The enzyme has a pI of 7.4-7.6, requires Mg2+, is partially stimulated by Mn2+, and is inhibited by EDTA. It has no detectable exonuclease or phosphomonoesterase activity. |
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Authors:
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N L Shaper; R H Grafstrom; L Grossman |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 257 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1982 Nov |
Date Detail:
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Created Date: 1983-01-07 Completed Date: 1983-01-07 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 13455-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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DNA-(Apurinic or Apyrimidinic Site) Lyase Deoxyribonuclease IV (Phage T4-Induced) Endodeoxyribonucleases / isolation & purification*, metabolism Female Humans Kinetics Molecular Weight Placenta / enzymology* Pregnancy Substrate Specificity |
| Grant Support | |
ID/Acronym/Agency:
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N01-CP-85670/CP/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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EC 3.1.-/Endodeoxyribonucleases; EC 3.1.21.2/Deoxyribonuclease IV (Phage T4-Induced); EC 4.2.99.18/DNA-(Apurinic or Apyrimidinic Site) Lyase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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