| Human placenta and fetal membranes express follistatin-related gene mRNA and protein. | |
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MedLine Citation:
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PMID: 14594115 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Activin A is a placental glycoprotein and possible biological actions during pregnancy, suggested by experimental data, are the modulation of cytotrophoblast differentiation, placental hormonogenesis and uterotonins secretion. Follistatin-related gene (FLRG) is a 70 amino acids protein which binds activin A with high affinity, and which modulates its biological effects on target tissues by preventing the activin A interaction with its receptors. The present study investigated whether trophoblast, decidua and fetal membranes express FLRG mRNA (by RT-PCR) and peptide (by immunohistochemistry). Tissue specimens were collected at first and third trimester of pregnancy, from patients undergoing voluntary pregnancy interruption (no.=6; from 8 to 12 gestational weeks) and elective caesarean section at term (no.=6; 39-40 weeks of pregnancy). FLRG mRNA was expressed by the various gestational tissues both at early gestation and at term pregnancy. Immunoreactive protein was found in the trophoblast cells, epithelial amniotic and chorionic cells and maternal decidua; nevertheless, the most intense FLRG stain was detected in the walls of decidual and placental blood vessels. In conclusion, FLRG mRNA and peptide are expressed by placenta and fetal membranes. Its different immunolocalization with respect to follistatin and activin A supports a different role for FLRG in modulating activin A actions into gestational tissues. |
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Authors:
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P Ciarmela; P Florio; P Toti; A Franchini; V Maguer-Satta; C Ginanneschi; E Ottaviani; F Petraglia |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of endocrinological investigation Volume: 26 ISSN: 0391-4097 ISO Abbreviation: J. Endocrinol. Invest. Publication Date: 2003 Jul |
Date Detail:
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Created Date: 2003-11-03 Completed Date: 2004-06-08 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 7806594 Medline TA: J Endocrinol Invest Country: Italy |
Other Details:
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Languages: eng Pagination: 641-5 Citation Subset: IM |
Affiliation:
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Department of Pediatrics, Obstetrics and Reproductive Medicine, University of Siena, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Chorionic Villi / metabolism Decidua / metabolism Extraembryonic Membranes / metabolism* Female Follistatin-Related Proteins / biosynthesis*, genetics* Gene Expression Regulation Humans Immunohistochemistry Placenta / metabolism* Pregnancy Pregnancy Trimester, First Pregnancy Trimester, Third RNA, Messenger / biosynthesis* Reverse Transcriptase Polymerase Chain Reaction Trophoblasts / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Follistatin-Related Proteins; 0/RNA, Messenger |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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