| Human peripheral blood mononuclear cells (PBMC) in early pregnancy promote embryo invasion in vitro: HCG enhances the effects of PBMC. | |
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MedLine Citation:
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PMID: 11756389 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The aim of this study was to investigate the role of peripheral blood mononuclear cells (PBMC) in embryo invasion at the implantation site and to estimate the effect on PBMC function of human chorionic gonadotrophin (HCG) that is secreted from the human embryo. METHODS AND RESULTS: The effect of PBMC on the invasiveness of murine embryos was examined using an invasion assay. PBMC obtained from women in early pregnancy (5-9 weeks gestation) significantly enhanced both spreading of murine embryos on Matrigel and invasion beneath the gel. These effects were greater than those of PBMC obtained from non-pregnant women in the secretory phase (cycle day 16-24) and the control (in the absence of PBMC). When PBMC obtained from non-pregnant women were incubated with recombinant HCG (10 IU/ml) for 2 days and were subjected to invasion assay using murine embryos, PBMC treated with HCG significantly promoted both spreading and invasion of murine embryos as compared with the non-treated PBMC. On the other hand, embryo outgrowth was not affected by HCG in the absence of PBMC, showing no direct effect of HCG on embryo invasion. CONCLUSION: This study indicated that PBMC from pregnant women promoted murine embryo invasion in vitro and this effect of PBMC was enhanced by HCG. These findings suggest that PBMC at the implantation site are activated by HCG secreted from the embryo, following which PBMC regulate embryo invasion. |
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Authors:
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Takahiro Nakayama; Hiroshi Fujiwara; Michiyuki Maeda; Takuya Inoue; Shinya Yoshioka; Takahide Mori; Shingo Fujii |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Human reproduction (Oxford, England) Volume: 17 ISSN: 0268-1161 ISO Abbreviation: Hum. Reprod. Publication Date: 2002 Jan |
Date Detail:
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Created Date: 2001-12-28 Completed Date: 2002-03-26 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8701199 Medline TA: Hum Reprod Country: England |
Other Details:
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Languages: eng Pagination: 207-12 Citation Subset: IM |
Affiliation:
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Department of Gynecology and Obstetrics, Faculty of Medicine, Kyoto University, Sakyo-ku, Japan. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blastocyst / drug effects, physiology Chorionic Gonadotropin / pharmacology* Coculture Techniques Collagen Drug Combinations Embryo Implantation* Embryo, Mammalian / physiology* Female Gestational Age* Humans Laminin Leukocytes, Mononuclear / physiology* Mice Pregnancy Proteoglycans Recombinant Proteins / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Chorionic Gonadotropin; 0/Drug Combinations; 0/Laminin; 0/Proteoglycans; 0/Recombinant Proteins; 119978-18-6/matrigel; 9007-34-5/Collagen |
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