| Human periodontal ligament cells reaction on a novel hydroxyapatite-collagen scaffold. | |
| | |
MedLine Citation:
|
PMID: 22681634 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
BACKGROUND: Periodontal tissue regeneration presents a highly promising method for restoring periodontal structures. The development of a suitable bioactive scaffold that promotes cell proliferation and differentiation is critical in periodontal tissue engineering. The aim of this study was to evaluate the biocompatibility of a novel 3-dimensional hydroxyapatite-collagen scaffold with human periodontal ligament (hPDL) cell culture. METHODS: The scaffold was produced from a natural collagen matrix - purified porcine acellular dermal matrix (PADM), which was then treated with hydroxyapatite (HA) through a biomimetic chemical process to obtain hydroxyapatite-porcine acellular dermal matrix (HA-PADM) scaffold. The hPDL cells were cultured with HA-PADM scaffolds for 1, 3, 6, 14, and 28 days. The cell viability assay, scanning electron microscopy (SEM), hematoxylin and eosin (H&E) staining, immunohistochemistry, and confocal microscopy were employed in different time points to evaluate the biocompatibility of the scaffolds with hPDL cells. RESULTS: The cell viability assay (WST-1 test) verified cell proliferation on the HA-PADM scaffolds. The SEM study showed unique morphology of hPDL cells, which attach and spread on the surface of the scaffolds. The H&E staining, immunohistochemistry, and confocal microscopy demonstrated that hPDL cells were able to grow into the HA-PADM scaffolds and maintain viability after prolonged culture. CONCLUSIONS: This study proved that HA-PADM scaffold is -biocompatible for hPDL cells. The cells were able to proliferate and migrate into the scaffold. These observations suggest that HA-PADM is a potential cell carrier for periodontal tissue regeneration. |
| | |
Authors:
|
Jing Guo; Ying Wang; Chengbo Cao; Rosemary Dziak; Brian Preston; Guoqiang Guan |
Related Documents
:
|
9057894 - Lead perturbs the regulation of spontaneous release of tissue plasminogen activator and... 2177954 - Stimulation of collagenase production by rat osteosarcoma cells can occur in a subpopul... 16739974 - Monitoring of glycoprotein products in cell culture lysates using lectin affinity chrom... 3138024 - Differential regulation of plasminogen activators by epidermal growth factor in normal ... 7310744 - Cell surface carbohydrates in tritrichomonas foetus. 19263264 - Cyclic rgd-targeting of reversibly stabilized dna nanoparticles enhances cell uptake an... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-6-8 |
Journal Detail:
|
Title: Dental traumatology : official publication of International Association for Dental Traumatology Volume: - ISSN: 1600-9657 ISO Abbreviation: - Publication Date: 2012 Jun |
Date Detail:
|
Created Date: 2012-6-11 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101091305 Medline TA: Dent Traumatol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
© 2012 John Wiley & Sons A/S. |
Affiliation:
|
Department of Orthodontics, School of Dental Medicine, University at Buffalo, State University of New York, Buffalo, NY, USA; Department of Orthodontics, School & Hospital of Stomatology, Shandong University, Jinan, Shandong, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Photoacoustic imaging of mesenchymal stem cells in living mice via silica-coated gold nanorods.
Next Document: A method for improved clustering and classification of microscopy images using quantitative co-local...