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Human periodontal ligament cells reaction on a novel hydroxyapatite-collagen scaffold.
MedLine Citation:
PMID:  22681634     Owner:  NLM     Status:  Publisher    
BACKGROUND: Periodontal tissue regeneration presents a highly promising method for restoring periodontal structures. The development of a suitable bioactive scaffold that promotes cell proliferation and differentiation is critical in periodontal tissue engineering. The aim of this study was to evaluate the biocompatibility of a novel 3-dimensional hydroxyapatite-collagen scaffold with human periodontal ligament (hPDL) cell culture. METHODS: The scaffold was produced from a natural collagen matrix - purified porcine acellular dermal matrix (PADM), which was then treated with hydroxyapatite (HA) through a biomimetic chemical process to obtain hydroxyapatite-porcine acellular dermal matrix (HA-PADM) scaffold. The hPDL cells were cultured with HA-PADM scaffolds for 1, 3, 6, 14, and 28 days. The cell viability assay, scanning electron microscopy (SEM), hematoxylin and eosin (H&E) staining, immunohistochemistry, and confocal microscopy were employed in different time points to evaluate the biocompatibility of the scaffolds with hPDL cells. RESULTS: The cell viability assay (WST-1 test) verified cell proliferation on the HA-PADM scaffolds. The SEM study showed unique morphology of hPDL cells, which attach and spread on the surface of the scaffolds. The H&E staining, immunohistochemistry, and confocal microscopy demonstrated that hPDL cells were able to grow into the HA-PADM scaffolds and maintain viability after prolonged culture. CONCLUSIONS: This study proved that HA-PADM scaffold is -biocompatible for hPDL cells. The cells were able to proliferate and migrate into the scaffold. These observations suggest that HA-PADM is a potential cell carrier for periodontal tissue regeneration.
Jing Guo; Ying Wang; Chengbo Cao; Rosemary Dziak; Brian Preston; Guoqiang Guan
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-8
Journal Detail:
Title:  Dental traumatology : official publication of International Association for Dental Traumatology     Volume:  -     ISSN:  1600-9657     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101091305     Medline TA:  Dent Traumatol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 John Wiley & Sons A/S.
Department of Orthodontics, School of Dental Medicine, University at Buffalo, State University of New York, Buffalo, NY, USA; Department of Orthodontics, School & Hospital of Stomatology, Shandong University, Jinan, Shandong, China.
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