Document Detail


Human parvovirus b19 infection in patients with coronary atherosclerosis.
MedLine Citation:
PMID:  20082878     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND AIMS: The identification of possible pathogens for an infectious etiology of atherosclerotic coronary artery disease (CAD) is an expanding field. The present study was undertaken to explore the role of parvovirus B19, a potent infectious agent. METHODS: A total of 565 patients were analyzed (90 patients with CAD, and 475 controls). Serologic analysis for human paravovirus B19 (B19) specific IgM and IgG was carried out in all patients. In addition, tissue specimens were obtained from five patients who received heart transplants. Direct in situ polymerase chain reaction (PCR) and immunocytochemistry were performed in the samples to localize B19 DNA. RESULTS: Enzyme immunoassay showed that the seropositive rate of anti-B19 IgG in patients with CAD was 1.5- to 2.7-fold more frequent than in healthy controls. Clinical characteristics did not affect the prevalence of seropositivity for B19. However, anti-B19 IgM and B19-specific DNA were not detected in healthy or individuals with CAD. Furthermore, nonradioactive in situ PCR found that the majority of B19-specific DNA was located in the endothelial cells of the thickened intima. CONCLUSIONS: Our results first demonstrate a seroepidemiological and histopathological association between chronic B19 infection and CAD, suggesting that B19 infection may have a potential role in the pathogenesis of coronary atherosclerosis.
Authors:
Shih-Chi Liu; Chia-Ti Tsai; Cho-Kai Wu; Meng-Fen Yu; Mu-Zon Wu; Liang-In Lin; Shoei-Shen Wang; Juey-Jen Hwang; Yung-Zu Tseng; Fu-Tien Chiang; Chuen-Den Tseng
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Archives of medical research     Volume:  40     ISSN:  1873-5487     ISO Abbreviation:  Arch. Med. Res.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2010-01-19     Completed Date:  2010-03-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9312706     Medline TA:  Arch Med Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  612-7     Citation Subset:  IM    
Copyright Information:
2009 IMSS. Published by Elsevier Inc.
Affiliation:
Min-Sheng Hospital, Tao-Yuan, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Antibodies, Viral / metabolism
Arteries / virology
Coronary Artery Disease* / etiology,  virology
DNA, Viral / metabolism
Humans
Immunoglobulin G / blood
Immunoglobulin M / blood
Male
Middle Aged
Parvoviridae Infections / blood,  complications*
Parvovirus B19, Human / genetics*,  immunology
Chemical
Reg. No./Substance:
0/Antibodies, Viral; 0/DNA, Viral; 0/Immunoglobulin G; 0/Immunoglobulin M

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