Document Detail


Human papillomavirus type 77 E7 protein is a weak deregulator of cell cycle.
MedLine Citation:
PMID:  16650526     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human Papillomavirus type 77 is a skin type found in non-melanoma skin cancers of immuno-compromised individuals. Although, the HPV77 E6 oncoprotein has been well studied, nothing is known about E7. Studies on mucosal HPV types (e.g. HPV16) showed that E7 deregulates the cell cycle by binding to and promoting degradation of retinoblastoma protein (pRb). Here, we characterized the impact of HPV77 E7 on the cell cycle. We observed that HPV77 E7 associated with pRb with a lower affinity than HPV16 E7, promoting weakly its degradation. Although, HPV16 E7 led to cellular proliferation and accumulation of the cell cycle inhibitor p16(INK4a), both events were not clearly observed in HPV77 E7 cells. Together, these data indicate that HPV77 E7 does not efficiently deregulate the cell cycle, in contrast to several E7s of mucosal HPV types.
Authors:
Mariam Mansour; Majid Touka; Adriana Malena; Cesare Indiveri; Wen Dong; Ilaria Gionfriddo; Rosita Accardi; Angelo Paradiso; Bakary S Sylla; Anne-Sophie Gabet; Massimo Tommasino
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-05-02
Journal Detail:
Title:  Cancer letters     Volume:  246     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2006-12-26     Completed Date:  2007-04-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  274-81     Citation Subset:  IM    
Affiliation:
International Agency for Research on Cancer, World Health Organization, Lyon, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Cycle / genetics,  physiology*
Cells, Cultured
Cyclin-Dependent Kinase Inhibitor p16 / genetics,  metabolism
Gene Expression
Genetic Vectors / genetics
Humans
Immunoblotting
Mice
NIH 3T3 Cells
Oncogene Proteins, Viral / genetics,  metabolism
Papillomavirus E7 Proteins / genetics,  metabolism*
Protein Binding
Retinoblastoma Protein / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Transfection
Chemical
Reg. No./Substance:
0/Cyclin-Dependent Kinase Inhibitor p16; 0/Oncogene Proteins, Viral; 0/Papillomavirus E7 Proteins; 0/Retinoblastoma Protein; 0/oncogene protein E7, Human papillomavirus type 16

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