Document Detail

Human papillomavirus infection in actinic keratosis and bowen's disease: comparative study with expression of cell-cycle regulatory proteins p21(Waf1/Cip1), p53, PCNA, Ki-67, and Bcl-2 in positive and negative lesions.
MedLine Citation:
PMID:  14562284     Owner:  NLM     Status:  MEDLINE    
We examined the presence of human papillomavirus (HPV) DNA in tissues of premalignant skin lesions, i.e., actinic keratosis (n = 13) and Bowen's disease (n = 62), taken from 69 Japanese immunocompetent and renal transplant recipient patients. Detection and typing of HPV DNA were performed using polymerase chain reaction (PCR) and sequence analysis or restriction fragment length polymorphism (RFLP) analysis, respectively. The positivity rates of HPV DNA in tissues of actinic keratosis and Bowen's disease were 77% and 65%, respectively. Twenty-seven HPV types were detected in 50 (67%) premalignant skin lesions, in which Z95963 (accession no. in the EMBL Databank), Z95968, AJ010823, and AJ000151 have been described as partial sequences of unknown HPV types. Furthermore, 2 unknown types, HPVX1 and HPVX2, were found in specimens of actinic keratosis. Sequence analysis showed that HPVX1 is related to HPV-37 (86.1% sequence homology) and that HPVX2 is related to HPV-38 (79.7%). These results indicate that various mucosal and epidermodysplasia verruciformis-related HPV types are associated with the pathogenesis of actinic keratosis and Bowen's disease. In addition, 24 specimens of HPV-positive or HPV-negative premalignant skin lesions were examined immunohistochemically for proliferating cells to determine biological differences between HPV-positive and HPV-negative lesions. Immunohistochemistry for p21(Waf1/Cip1), p53, proliferating cell nuclear antigen (PCNA), Ki-67, and Bcl-2 revealed that there was no significant difference in the cell proliferation activity between HPV-positive and HPV-negative lesions, suggesting that HPV infection alone does not induce cell proliferation in those lesions.
Tsuyoshi Mitsuishi; Seiji Kawana; Toshihiko Kato; Makoto Kawashima
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Human pathology     Volume:  34     ISSN:  0046-8177     ISO Abbreviation:  Hum. Pathol.     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-10-16     Completed Date:  2003-10-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9421547     Medline TA:  Hum Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  886-92     Citation Subset:  IM    
Department of Dermatology, Nippon Medical School, Tokyo, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AJ000151;  AJ010823;  Z95968
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MeSH Terms
Aged, 80 and over
Base Sequence
Bowen's Disease / metabolism,  pathology,  virology*
Cell Cycle Proteins / metabolism*
DNA, Viral / analysis
Keratosis / metabolism,  pathology,  virology*
Middle Aged
Molecular Sequence Data
Papillomaviridae / genetics,  isolation & purification,  pathogenicity
Papillomavirus Infections / complications*,  metabolism,  pathology
Photosensitivity Disorders / metabolism,  pathology,  virology*
Skin Neoplasms / metabolism,  pathology,  virology*
Tumor Virus Infections / complications*,  metabolism,  pathology
Reg. No./Substance:
0/Cell Cycle Proteins; 0/DNA, Viral

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