Document Detail

Human papillomavirus genotypes detected in clinician-collected and self-collected specimens from women living in the Mississippi Delta.
Jump to Full Text
MedLine Citation:
PMID:  23289357     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: There are no data available on human papillomavirus (HPV) infections in women living in the Mississippi Delta, where cervical cancer incidence and mortality among African American women is among the highest in the United States. The aim of this analysis was to report the age-specific prevalence of HPV in this population.
METHODS: We recruited 443 women, 26-65 years of age, from the general population of women living in the Mississippi Delta to participate; 252 women had been screened for cervical cancer within the last 3 years while 191 had not. Women underwent a pelvic exam and had clinician-collected Pap sample taken for the routine cervical cancer screening by cytology. Women were asked to collect a self-collected specimen at home and return it to the clinic. Both specimens were tested for HPV genotypes.
RESULTS: Four hundred and six women (91.6%) had HPV genotyping results for the clinician-collected and self-collected specimens. The prevalence of carcinogenic HPV was 18.0% (95% CI: 14.4%-22.1%) for clinician-collected specimens and 26.8% (95% CI: 22.6%-31.4%) for self-collected specimens. The concordance for the detection of carcinogenic HPV between clinician-collected and self-collected specimens was only fair (kappa = 0.54). While the prevalence of carcinogenic HPV in either sample decreased sharply with increasing age (ptrend< 0.01), the prevalence of non-carcinogenic HPV did not, especially the prevalence of HPV genotypes in the alpha 3/4/15 phylogenetic group.
CONCLUSIONS: The prevalence of carcinogenic HPV in our sample of women living in the Mississippi Delta was greater than the prevalence reported in several other U.S. studies. The high carriage of HPV infection, along with lack of participation in cervical cancer screening by some women, may contribute to the high cervical cancer burden in the region.
Philip E Castle; Julia C Gage; Edward E Partridge; Alfio Rausa; Patti E Gravitt; Isabel C Scarinci
Related Documents :
23796457 - Racial and ethnic variations in the effects of family history of colorectal cancer on s...
23395457 - Clinical utility of n-terminal pro-b-type natriuretic peptide for risk stratification o...
22925207 - The association between female genital mutilation and intimate partner violence.
23826627 - Disparities in reported psychosocial assessment across public and private maternity set...
22238467 - Risk factors for incident condyloma in a multinational cohort of men: the him study.
11919497 - Hiv-1 in ethnic shan migrant workers in northern thailand.
Publication Detail:
Type:  Journal Article     Date:  2013-01-07
Journal Detail:
Title:  BMC infectious diseases     Volume:  13     ISSN:  1471-2334     ISO Abbreviation:  BMC Infect. Dis.     Publication Date:  2013  
Date Detail:
Created Date:  2013-02-12     Completed Date:  2013-06-18     Revised Date:  2014-11-13    
Medline Journal Info:
Nlm Unique ID:  100968551     Medline TA:  BMC Infect Dis     Country:  England    
Other Details:
Languages:  eng     Pagination:  5     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Age Factors
Early Detection of Cancer
Mass Screening
Middle Aged
Papillomaviridae / classification,  genetics*
Papillomavirus Infections / diagnosis*,  epidemiology
Specimen Handling
Uterine Cervical Neoplasms / diagnosis,  epidemiology,  virology
Vaginal Smears

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): BMC Infect Dis
Journal ID (iso-abbrev): BMC Infect. Dis
ISSN: 1471-2334
Publisher: BioMed Central
Article Information
Download PDF
Copyright ©2013 Castle et al.; licensee BioMed Central Ltd.
Received Day: 14 Month: 6 Year: 2012
Accepted Day: 19 Month: 12 Year: 2012
collection publication date: Year: 2013
Electronic publication date: Day: 7 Month: 1 Year: 2013
Volume: 13First Page: 5 Last Page: 5
PubMed Id: 23289357
ID: 3570306
Publisher Id: 1471-2334-13-5
DOI: 10.1186/1471-2334-13-5

Human papillomavirus genotypes detected in clinician-collected and self-collected specimens from women living in the Mississippi Delta
Philip E Castle1 Email:
Julia C Gage2 Email:
Edward E Partridge3 Email:
Alfio Rausa4 Email:
Patti E Gravitt5 Email:
Isabel C Scarinci3 Email:
1Global Cancer Imitative, Chestertown, MD, USA
2Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, DHHS, Bethesda, MD, USA
3University of Alabama at Birmingham, Birmingham, AL, USA
4Mississippi State Department of Health, Jackson, MS, USA
5Department of Epidemiology, Johns Hopkins School of Public Health, Baltimore, MD, USA


Persistent cervical HPV infection by cancer-associated or carcinogenic HPV is the necessary cause of cervical cancer [1,2]. DNA testing for HPV is more sensitive but less specific for detection of precancerous lesions and early cancer than cervical cytology [3-5]. HPV DNA testing of self-collected specimens is equally sensitive as cytology [6] and could be used to reach populations not being screened by current programs [7-15].

The Mississippi Delta is a little studied population that has historically had several-fold higher annual rates of cervical cancer incidence and mortality than those for the general U.S. [16,17]. The overarching goal of our study in the Mississippi Delta was to study the acceptability of self-collection and HPV testing as an alternative to Pap testing. We previously reported that under-screened women were almost twice as likely to choose self-collection over free Pap testing, and twice as many of those who chose self-collection completed their screening compared those who chose free Pap testing [18]. The goal of this specific analysis was examine the impact of age and anatomic site of specimen collection on the prevalence of HPV, as a proxy for specificity. We wanted to understand the potential impact of using self-collection and HPV DNA testing on the patient.


Recruitment and clinical methods were detailed previously [19]. Briefly, women undergoing routine screening (n = 252)(“screened”) or had not been screened in the last three years (n = 191) (“under-screened”) were recruited as part of a larger study of self-collection and HPV DNA testing in 4 counties (Tallahatchie, Leflore, Sunflower, and Washington) located in northwest Mississippi and part of the Mississippi Delta. Women aged 26 to 65 years of age, non-pregnant, with a cervix, and willing to provide written, informed consent were enrolled into the study. Institutional review boards from the NCI, UAB, State of Mississippi Health Department, and Westat approved this study.

For all participants, cervical specimens were collected into PreservCyt (Hologic, Bedford, MA, USA) for routine cervical cytology and the residual was retained for HPV testing. Women were given a kit for self-collection at home, including a self-collection device [20,21] (,475,165.PN.&OS=PN/6,475,165&RS=PN/6,475,165) and a vial of Scope™ (Proctor and Gamble, Cincinnati, OH, USA) mouthwash for specimen transport [22]. The self-collection device physically and mechanically resembles a tampon and includes an outer sheath to shield the collection from vaginal contamination and irrelevant vaginal HPV infections. Women were instructed to insert the device into their anterior vagina, expose the Dacron collection tip to obtain a sample of cervical cells, retract the tip into the sheath, remove the device, and eject the tip into the transport medium. We used mouthwash as our transport medium to avoid giving kits containing toxic liquid-based cytology medium to our participants to take home [22].

Specimens were tested for 37 HPV genotypes (6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 45, 51–56, 58, 59, 61, 62, 64, 66–73, 81–84, 82v, and 89) using Linear Array (LA; Roche Molecular Systems, Pleasanton, CA, USA) [23,24]. HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, and 68 were classified as the certain or probable carcinogenic HPV genotypes [25]. HPV genotypes were also classified according to broad branches in the phylogenetic tree for HPV genotypes [26,27]: 1) HPV genotypes 6, 11, 40, and 42, which are classified in the alpha 1, 8, and 10 group (alpha 1/8/10 genotypes). HPV 6 and 11 cause 90% of condyloma accuminata (genital warts); 2) HPV genotypes 16, 18, 26, 31, 33, 35, 39, 45, 51–56, 58, 59, 64, 66, 67, 68, 69, 70, 73, 82, and 82v, which are classified in the alpha 5, 6, 7, 9, and 11 group (alpha 5/6/7/9/11 genotypes). All the carcinogenic and borderline carcinogenic HPV genotypes are found in these species; and 3) HPV genotypes 61, 62, 71, 72, 81, 83, 84, and 89, which are classified in the alpha 3, 4, and 15 group (alpha 3/4/15 genotypes). There is some evidence that these HPV genotypes in these species have a predilection for vaginal tissue [28-31].

Logistic regression was used to calculate odds ratios (OR), 95% confidence intervals, and p values to test for differences between study groups (screened vs. under-screened) and linear trends with age groups (26–39, 30–39, 40–49, and 50–65 years) for testing HPV positive for categories of HPV. For categories of HPV prevalences, both crude and adjusted for study group and age group, were calculated. Binomial exact 95% confidence intervals were used where noted. An exact version of McNemar chi-square test or a symmetry chi-square test was used to test for differences in prevalence for individual or specific group of HPV genotypes from the same specimen or for same individual or specific group of HPV genotypes from different specimens.

A post-hoc power calculation based on a McNemar chi-square test indicated that 1) a sample size of 406 pairs for the whole analysis population achieves 80% power (alpha = 0.05) to detect a difference in prevalence of 4.5% between specimens collections if the discordance was 10%, of 6.4% if the discordance was 20%, and of 7.8% if the discordance was 30%, and 2) a sample size of 312 pairs for women 30 years and older achieves 80% power (alpha = 0.05) to detect a difference in prevalence of 5.2% between specimens collections if the discordance was 10%, of 7.3% if the discordance was 20%, and 8.9% if the discordance was 30%.

A p value of < 0.05 was considered statistically significant. STATA Version 11.1 was used for these analyses (StataCorp, College Station, Texas, USA).


There were HPV testing results available for 440 (99.7%) clinician-collected specimens and 409 (92.3%) self-collected specimens. The analyses were restricted to the 406 (91.6%) women for whom both results were available, which included 231 screened women and 175 under-screened women. Of the 406 subjects included in this analysis, 370 (91.1%) identified themselves as Black/African American, 33 (8.1%) as White/Caucasian, and 3 (0.7%) as other. The mean and median ages were 37.5 years and 36 years, respectively.

We used logistic regression to show the impact of the two study groups and age on detection of HPV. As shown in Table 1, there was no significant difference in the HPV detection between study populations for categories of any HPV, carcinogenic HPV, non-carcinogenic HPV, and three phylogenetically defined categories of alpha 1/8/10, alpha 5/6/7/9/11, and alpha 3/4/15 HPV genotypes between populations for clinician-collected specimens or for self-collected specimens. We noted that in general the under-screened population had more HPV, albeit not significantly so; underscreened women were non-significantly more likely to have alpha 1/8/10 genotypes compared to screened women (OR = 1.9, 95% CI: 0.74-4.7). Because there was no evidence of significant differences in the prevalence of HPV between the screened and under-screened groups, the two were combined henceforth.

Increasing age was statistically associated with a lower likelihood of testing positive of any HPV, carcinogenic HPV, and alpha 5/6/7/9/11 genotypes, but not for alpha 3/4/15 genotypes, for both specimen types (Table 1).

We calculated the prevalence of HPV categories by collection method, and the concordance between specimens, as shown in Table 2; prevalence adjusted for age group and the population group (screened vs. under-screened) was not appreciably different (data not shown). The prevalence of any HPV was 42.4% (95% CI: 37.5%-47.3%) for clinician-collected specimens and 54.7% (95% CI: 49.7%-59.6%) for self-collected specimens; the discordance (disagreement) was 27% and therefore the post-hoc power was 99.7%. The adjusted prevalence of carcinogenic HPV was 18.0% (95% CI: 14.4%-22.1%) for clinician-collected specimens and 26.8% (95% CI: 22.6%-31.4%) for self-collected specimens. Self-collected specimens were more likely to test positive for any HPV, carcinogenic HPV, non-carcinogenic HPV, alpha 5/6/7/9/11, and alpha 3/4/15 genotypes than clinician-collected specimens (p < 0.001). As a consequence of the differences in HPV detection by specimen type, the concordance between the two specimens for HPV detection in any HPV category was only fair, with kappa values around 0.50.

The prevalence of carcinogenic HPV for women 30 and older (n = 312), the ages at which HPV and cytology cotesting is acceptable by national screening guidelines [32-34], was 14.4% (95% CI: 10.7%-18.8%) for clinician-collected specimens and 24.0% (95% CI: 19.4%-29.2%) for self-collected specimens, the latter of which was significantly greater than the former (p < 0.001). The discordance (disagreement) was 17% and therefore the post-hoc power was 98.7%.

The concordance for HPV detection, categorizing hierarchically according to cancer risk, for the two specimens is shown in Table 3. The crude kappa was 0.47 and the percentage agreement was 66%. There was a significant difference in detection of HPV classified in this manner (p = 0.0002), primarily because the following discordant (self-collected/clinician-collected) results were common: 1) non-carcinogenic HPV/HPV negative; 2) carcinogenic HPV/HPV negative; and 3) carcinogenic HPV/non-carcinogenic HPV.

The agreement statistics for detection of all 37 HPV genotypes individually in self-collected vs. clinician-collected specimens were a kappa value of 0.56 (95% CI: 0.52-0.61), the total agreement of 97.8%, and the positive agreement of 40.0%, with self-collected specimens more likely to test positive for any of the HPV genotypes than clinician-collected specimens (p < 0.0001). The prevalence for individual HPV genotypes is shown in the Additional file 1: Table S1. The 5 most common HPV genotypes found in clinician-collected specimens were HPV54 (4.9%), HPV62 (4.7%), HPV83 (4.4%), HPV52 (3.9%), and HPV71 (3.4%); prevalences of HPV16 and HPV18, the two genotypes targeted by the current generation of HPV vaccines were 2.5% and 1.5%, respectively. The 5 most common HPV genotypes found in self-collected specimens were HPV54 (8.1%), HPV83 (7.9%), HPV70 (7.4%), HPV62 (7.4%), and HPV81 (6.4%); the prevalences of HPV16 and HPV18, the two genotypes targeted by the current generation of HPV vaccines were 3.0% and 3.9%, respectively. Individually, HPV18, 33, 54, 55, 62, 68, 70, 81, 83, and 84 were more commonly detected (p < 0.05) in self-collected specimens than clinician-collected specimens.

Shown in Figure 1 are the age group-specific patterns of prevalences for any HPV, any carcinogenic HPV, alpha 5/6/7/9/11 genotypes, and any alpha 3/4/15 genotypes for the two groups combined. While the prevalence of carcinogenic HPV detected in clinician-collected (ptrend = 0.0002) and self-collected (ptrend = 0.009) specimens decreased with increasing age, the prevalence of non-carcinogenic HPV did not (ptrend = 0.3 for clinician-collected, ptrend = 0.08 for self-collected). Grouping of HPV genotypes based on branches in the phylogenetic tree highlighted these differences in age trends. While the prevalence of alpha 5/6/7/9/11 detected in clinician-collected (ptrend = 0.001) and self-collected (ptrend = 0.007) decreased with increasing age, the prevalence of alpha 3/4/15 did not (ptrend = 0.4 for clinician-collected, ptrend = 0.4 for self-collected).


We found the prevalence of carcinogenic HPV to be relatively high in this population compared to other U.S. populations. The prevalence of carcinogenic HPV in clinician-collected specimens from women 30 and older in this population was approximately 2-fold higher than what has been observed at Kaiser Permanente Northern California (6.5% in women 30 and older) [35], and 40% higher in both a study that included women attending sexually transmitted infection (STI) clinics (~10% in women 30 and older) [36] and in a state-wide population study of women living in New Mexico (~10% in women 31 and older) [37], a state that ranks along with Mississippi as one of the poorest states in the US. The high prevalence of carcinogenic HPV reported in this study was not due to the use of LA for HPV detection as the prevalence of HPV was similar using Hybrid Capture 2 (p = 0.9) [19] the test used in these other reports in two of the aforementioned studies (35;36). We speculate that the higher prevalence of carcinogenic HPV, which has been shown to correlate with cervical cancer incidence [38], and a lack of screening in a subset of women living in the Mississippi Delta may in part account for the higher annual rate of cervical cancer incidence in this region compared to other places in the U.S. [16].

We note that this study was only cross-sectional and therefore could not assess the total lifetime exposure to HPV, nor could we measure HPV persistence, which precedes and predicts the development of cervical precancerous lesions [39]. Yet HPV prevalence has been correlated with the risk of cervical cancer [38], supporting the possibility that that this population of women living in the Mississippi Delta are at a higher risk than the general U.S. population.

The prevalence of HPV and the HPV genotypes detected was strongly influenced by both the age of the patient and the type of sampling (clinician vs. self). The higher prevalence of carcinogenic HPV in women under 30 (vs. 30 and older) suggests may preclude the use of self-collection and HPV testing in these younger women although the decision to use it has to be weighed against the possibility that these higher-risk women may not get the screening they need in the future.

While we did not observe an increase in alpha 3/4/15 genotypes in cervical specimens with age as observed in other studies (29;30), the prevalence of these HPV genotypes remained relatively unchanged with increasing age. As a consequence, the proportion of alpha 3/4/15 genotypes in the cervical specimens among any HPV infections increased significantly with age so that the prevalence of alpha 5/6/7/9/11 and alpha 3/4/15 genotypes were approximately the same in women 50 and older. To our surprise, the age pattern in the vagina as measured by self-collection was similar to that of the cervix, with higher prevalence of alpha 5/6/7/9/11 genotypes than alpha 3/4/15 genotypes in young women rather than the preponderance of alpha 3/4/15 genotypes at all ages. The differences in this study versus other studies could be due to the relative small sample size in this study or unmeasured differences in sexual behaviors. Another possibility is that self-collection device used in this study did a better job of sampling the cervix, making the self-collected specimens more representative of the cervical milieu of HPV genotypes than observed in other studies.

We note that the one of our limitations for this study is that we used non-FDA approved HPV test, self-collection device, and transport medium. Linear Array, the HPV test used in this study, is one of the standard methods for HPV genotyping [40] and has been shown to correlate well with Hybrid Capture 2 [24,41] and cobas4800 [42], two FDA-approved tests. There is no collection device, including tampons and collection brushes and brooms, which is FDA approved for self-collection. Finally, mouthwash has been used for genetic (DNA) testing for epidemiologic studies, including those that send specimens through the mail [43]. Here, we applied it to HPV DNA rather than host genomic DNA. Nevertheless, the results of this analysis should be considered in relative rather than absolute terms since it is possible that that the methods in this study led to false positive and/or false negative results.


Our data illustrated how self-collected specimens can result in significantly higher point prevalence of HPV (lower specificity), including carcinogenic HPV, than a cervical specimen. The point prevalence of HPV using self-collected specimens in this study was comparable to what was recently reported [44] in a nationally representative HPV survey using cervicovaginal lavages tested with the same HPV genotyping assay. On one hand, the self-collected cervicovaginal specimens, with the higher point prevalence, may be more representative of the total lower female genital tract burden to HPV than samples taken only from the cervix. Self-collection, despite the increased detection of carcinogenic HPV, is not as sensitive as a clinician-collection for detecting prevalent cervical precancer or cancer [6]. However, it is unknown whether added detection of vaginal carcinogenic HPV not found at the cervix is in anyway associated with future risk of the cervical or lower genital tract HPV-related cancer.

Competing interests

Dr. Castle serves on a data and safety monitoring board for Merck. He has received HPV tests and testing for research at a reduced or no cost from Qiagen Roche Norchip, and MTM. Dr. Castle has served as a paid consultant to BD, Cepheid, and GE Healthcare and has received an honorarium for speaking from Roche.

Authors’ contributions

PEC participated in the design and oversaw the execution of the study, conducted the primary analysis, and drafted the manuscript. JCG assisted in the analysis and the drafted the manuscript. EEP participated in the design of the study and the conduct of the study. AR participated in the design and was responsible for the clinical care of the patients. PEG did the HPV testing and helped to draft the manuscript. ICS participated in the design and oversaw the execution of the study, and helped to draft the manuscript. All authors read and approved the final manuscript.

The intramural research program of the NIH/NCI and the NCI’s Center for Reducing Cancer Health Disparities supported this work.

Pre-publication history

The pre-publication history for this paper can be accessed here:

Supplementary Material Additional file 1: Table S1

The prevalence of 37 HPV genotypes in clinician-collected and self-collected specimens. Bold type indicates statistically greater prevalence of that type as determined using an exact version of the McNemar’s tests.

Click here for additional data file (1471-2334-13-5-S1.ppt)

Schiffman M,Castle PE,Jeronimo J,et al. Human papillomavirus and cervical cancerLancetYear: 200737089090710.1016/S0140-6736(07)61416-017826171
Walboomers JM,Jacobs MV,Manos MM,et al. Human papillomavirus is a necessary cause of invasive cervical cancer worldwideJ PatholYear: 1999189121910.1002/(SICI)1096-9896(199909)189:1<12::AID-PATH431>3.0.CO;2-F10451482
Naucler P,Ryd W,Tornberg S,et al. Human papillomavirus and Papanicolaou tests to screen for cervical cancerN Engl J MedYear: 20073571589159710.1056/NEJMoa07320417942872
Mayrand MH,Duarte-Franco E,Rodrigues I,et al. Human papillomavirus DNA versus Papanicolaou screening tests for cervical cancerN Engl J MedYear: 20073571579158810.1056/NEJMoa07143017942871
Cuzick J,Clavel C,Petry KU,et al. Overview of the European and North American studies on HPV testing in primary cervical cancer screeningInt J CancerYear: 20061191095110110.1002/ijc.2195516586444
Zhao FH,Lewkowitz AK,Chen F,et al. Pooled analysis of a self-sampling HPV DNA Test as a cervical cancer primary screening methodJ Natl Cancer InstYear: 201210417818810.1093/jnci/djr53222271765
Harper DM,Hildesheim A,Cobb JL,et al. Collection devices for human papillomavirusJ Fam PractYear: 19994853153510428251
Virtanen A,Nieminen P,Luostarinen T,et al. Self-sample HPV tests as an intervention for nonattendees of cervical cancer screening in Finland: a randomized trialCancer Epidemiol Biomarkers PrevYear: 2011201960196910.1158/1055-9965.EPI-11-030721752985
Gok M,Heideman DA,van Kemenade FJ,et al. HPV testing on self collected cervicovaginal lavage specimens as screening method for women who do not attend cervical screening: cohort studyBMJYear: 2010340c104010.1136/bmj.c1040.:c104020223872
Gok M,Heideman DA,van Kemenade FJ,et al. Offering self-sampling for human papillomavirus testing to non-attendees of the cervical screening programme: Characteristics of the respondersEur J CancerYear: 2012481799180810.1016/j.ejca.2011.11.02222172570
Szarewski A,Cadman L,Mesher D,et al. HPV self-sampling as an alternative strategy in non-attenders for cervical screening - a randomised controlled trialBr J CancerYear: 201110491592010.1038/bjc.2011.4821343937
Lindell M,Sanner K,Wikstrom I,et al. Self-sampling of vaginal fluid and high-risk human papillomavirus testing in women aged 50 years or older not attending Papanicolaou smear screeningBJOGYear: 201211924524822017806
Harper DM,Longacre MR,Noll WW,et al. Factors affecting the detection rate of human papillomavirusAnn Fam MedYear: 2003122122710.1370/afm.9015055412
Harper DM,Noll WW,Belloni DR,et al. Randomized clinical trial of PCR-determined human papillomavirus detection methods: self-sampling versus clinician-directed–biologic concordance and women’s preferencesAm J Obstet GynecolYear: 200218636537310.1067/mob.2002.12107611904593
Harper DM,Raymond M,Noll WW,et al. Tampon samplings with longer cervicovaginal cell exposures are equivalent to two consecutive swabs for the detection of high-risk human papillomavirusSex Transm DisYear: 20022962863610.1097/00007435-200211000-0000312438897
Freeman HP,Wingrove BK,Excess Cervical Cancer Mortality: A Marker for Low Access to Health Care in Poor CommunitiesYear: 2005Rockville, MD: National Cancer Institute, Center to Reduce Cancer Health Disparities NIH Pub. No. 05–5282.
Scarinci IC,Garcia FA,Kobetz E,et al. Cervical cancer prevention: new tools and old barriersCancerYear: 20101162531254220310056
Castle PE,Rausa A,Walls T,et al. Comparative community outreach to increase cervical cancer screening in the Mississippi DeltaPrev MedYear: 20115245245510.1016/j.ypmed.2011.03.01821497619
Gage JC,Partridge EE,Rausa A,et al. Comparative performance of human papillomavirus DNA testing using novel sample collection methodsJ Clin MicrobiolYear: 2011494185418910.1128/JCM.01254-1121998422
Castle PE,Aftab A,Saint-Jean G,et al. Detection of carcinogenic human papillomavirus in specimens collected with a novel self-sampling deviceJ Clin MicrobiolYear: 2006442158215910.1128/JCM.02358-0516757614
Kobetz E,Kish JK,Campos NG,et al. Burden of Human Papillomavirus among Haitian Immigrants in Miami, Florida: Community-Based Participatory Research in ActionJ OncolYear: 20122012728397 Epub;%2012 Mar 15.:728397. 22619675
Castle PE,Sadorra M,Garcia FA,et al. Mouthwash as a low-cost and safe specimen transport medium for human papillomavirus DNA testing of cervicovaginal specimensCancer Epidemiol Biomarkers PrevYear: 20071684084310.1158/1055-9965.EPI-06-090917416781
Castle PE,Gravitt PE,Solomon D,et al. Comparison of linear array and line blot assay for detection of human papillomavirus and diagnosis of cervical precancer and cancer in the atypical squamous cell of undetermined significance and low-grade squamous intraepithelial lesion triage studyJ Clin MicrobiolYear: 20084610911710.1128/JCM.01667-0717989194
Gravitt PE,Schiffman M,Solomon D,et al. A comparison of linear array and hybrid capture 2 for detection of carcinogenic human papillomavirus and cervical precancer in ASCUS-LSIL triage studyCancer Epidemiol Biomarkers PrevYear: 2008171248125410.1158/1055-9965.EPI-07-290418483347
Bouvard V,Baan R,Straif K,et al. A review of human carcinogens–Part B: biological agentsLancet OncolYear: 20091032132210.1016/S1470-2045(09)70096-819350698
Schiffman M,Herrero R,Desalle R,et al. The carcinogenicity of human papillomavirus types reflects viral evolutionVirologyYear: 200520337768415914222
Bernard HU,Burk RD,Chen Z,et al. Classification of papillomaviruses (PVs) based on 189 PV types and proposal of taxonomic amendmentsVirologyYear: 2010401707910.1016/j.virol.2010.02.00220206957
Castle PE,Schiffman M,Bratti MC,et al. A population-based study of vaginal human papillomavirus infection in hysterectomized womenJ Infect DisYear: 200419045846710.1086/42191615243917
Castle PE,Rodriguez AC,Porras C,et al. A comparison of cervical and vaginal human papillomavirusSex Transm DisYear: 20073484985510.1097/OLQ.0b013e318064c8c517621246
Castle PE,Jeronimo J,Schiffman M,et al. Age-related changes of the cervix influence human papillomavirus type distributionCancer ResYear: 2006661218122410.1158/0008-5472.CAN-05-306616424061
Castle PE,Schiffman M,Glass AG,et al. Human papillomavirus prevalence in women who have and have not undergone hysterectomiesJ Infect DisYear: 20061941702170510.1086/50951117109342
Saslow D,Solomon D,Lawson HW,et al. American Cancer Society, American Society for Colposcopy and Cervical Pathology, and American Society for Clinical Pathology screening guidelines for the prevention and early detection of cervical cancerCA Cancer J ClinYear: 20126214717210.3322/caac.2113922422631
Saslow D,Solomon D,Lawson HW,et al. American Cancer Society, American Society for Colposcopy and Cervical Pathology, and American Society for Clinical Pathology Screening Guidelines for the Prevention and Early Detection of Cervical CancerJ Low Genit Tract DisYear: 20121617520410.1097/LGT.0b013e31824ca9d522418039
Moyer VA,Screening for Cervical Cancer: U.S. Preventive Services Task Force Recommendation StatementAnn Intern MedYear: 201215688089122711081
Castle PE,Fetterman B,Poitras N,et al. Five-year experience of human papillomavirus DNA and papanicolaou test cotestingObstet GynecolYear: 200911359560019300322
Datta SD,Koutsky LA,Ratelle S,et al. Human papillomavirus infection and cervical cytology in women screened for cervical cancer in the United States, 2003–2005Ann Intern MedYear: 200814849350018378945
Wheeler CM,Hunt WC,Cuzick J,et al. A Population-based study of HPV genotype prevalence in the United States: Baseline measures prior to mass HPV vaccinationInt J CancerYear: 201313219820710.1002/ijc.2760822532127
Maucort-Boulch D,Franceschi S,Plummer M,International correlation between human papillomavirus prevalence and cervical cancer incidenceCancer Epidemiol Biomarkers PrevYear: 20081771772010.1158/1055-9965.EPI-07-269118349293
Wright TC Jr,Schiffman M,Adding a test for human papillomavirus DNA to cervical-cancer screeningN Engl J MedYear: 200334848949010.1056/NEJMp02017812571255
Castle PE,Porras C,Quint WG,et al. Comparison of two PCR-based human papillomavirus genotyping methodsJ Clin MicrobiolYear: 2008463437344510.1128/JCM.00620-0818716224
Stevens MP,Garland SM,Rudland E,et al. Comparison of the Digene Hybrid Capture 2 assay and Roche AMPLICOR and LINEAR ARRAY human papillomavirus (HPV) tests in detecting high-risk HPV genotypes in specimens from women with previous abnormal Pap smear resultsJ Clin MicrobiolYear: 2007452130213710.1128/JCM.02438-0617494721
Castle PE,Sadorra M,Lau T,et al. Evaluation of a prototype real-time PCR assay for carcinogenic human papillomavirus (HPV) detection and simultaneous HPV genotype 16 (HPV16) and HPV18 genotypingJ Clin MicrobiolYear: 2009473344334710.1128/JCM.00725-0919675214
Garcia-Closas M,Egan KM,Abruzzo J,et al. Collection of genomic DNA from adults in epidemiological studies by buccal cytobrush and mouthwashCancer Epidemiol Biomarkers PrevYear: 20011068769611401920
Hariri S,Unger ER,Sternberg M,et al. Prevalence of genital human papillomavirus among females in the United States, the National Health And Nutrition Examination Survey, 2003–2006J Infect DisYear: 201120456657310.1093/infdis/jir34121791659


[Figure ID: F1]
Figure 1 

The age group-specific prevalence of any (A), any carcinogenic (HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, and 68) (B), any alpha 5/6/7/9/11 (HPV16, 18, 26, 31, 33, 35, 39, 45, 51-56, 58, 59, 64, 66, 67, 68, 69, 70, 73, 82, and 82v) (C), and alpha 3/4/15 (HPV 61, 62, 71, 72, 81, 83, 84, and 89) (D) human papillomavirus (HPV) types as detected in clinician-collected cervical specimens and self-collected cervicovaginal specimens. Age groups were 26-29 (white bars), 30-39 (light gray bars) , 40-49 (medium gray bars), and 50-65 (black bars) years old (y.o.)

[TableWrap ID: T1] Table 1 

Results of logistic regression models to examine the relationship of study population and age on detection of HPV groups

Clinician collected
  OR p 95% CI ptrend OR p 95% CI ptrend
alpha 1/8/10
alpha 5/6/7/9/11
alpha 3/4/15 1.1 0.8 0.64-1.8 0.4 0.79 0.3 0.51-1.2 0.7

Odds ratios (OR) with 95% confidence intervals (95%CI) were calculated as a measure of association of women not having been screened in the last 3 years (vs. the reference of those who had) with HPV detection; p value was included for reference. Age groups were modeled continuously to assess the age trend (ptrend).

[TableWrap ID: T2] Table 2 

Prevalence of HPV groups in clinician- and self-collected specimens (n = 406)

% Positive agreement  
  Prevalence 95% CI Prevalence 95% CI % Agreement Kappa p  
alpha 1/8/10
alpha 5/6/9/11
alpha 3/4/15 21.2% 17.3%-25.5% 31.8% 27.3%-36.5% 82% 48% 0.53 <0.001

Differences in prevalence detected in clinician- and self-collected specimens were tested for statistical differences using an exact version of McNemar’s chi-square test. P value of less than 0.05 is considered significant and indicated by bolded font.

[TableWrap ID: T3] Table 3 

A comparison of detection of human papillomavirus (HPV) genotypes, classified hierarchically according to cancer risk (HPV16>HPV18>other carcinogenic HPV>non-carcinogenic HPV>HPV negative), for clinician-collected and self-collected specimens from women living in the Mississippi Delta

Self collected
    HPV16 HPV18 Other Carc. Non Carc. HPV Negative Total
Clinician Collected
HPV Negative
% cell
Non Carcinogenic
% cell
Other Carcinogenic
% cell
% cell
% cell
Total   12 14 83 113 184 406

The number (n) and cell percentage (% cell) for each pairwise results is shown. The crude kappa was 0.47, percent agreement was 66%, and symmetry chi-square was p = 0.0002. Abbreviation: carc., carcinogenic.

Article Categories:
  • Research Article

Keywords: Human papillomavirus (HPV), Self-collection, Pap, Cervical intraepithelial neoplasia, Cervix.

Previous Document:  [i]Vipera berus[/i] bites in Eastern Poland - a retrospective analysis of 15 case studies.
Next Document:  Oral clofarabine for relapsed/refractory non-Hodgkin lymphomas: results of a phase 1 study.