Document Detail


Human papillomavirus (HPV), DNA aberrations and cell cycle progression in anal squamous cell carcinoma patients.
MedLine Citation:
PMID:  18214063     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human papillomavirus (HPV) infections of the genital tract are sexually transmitted and prevalent worldwide. In this study, the role of HPV in 72 patients with anal squamous cell carcinoma was investigated. PATIENTS AND METHODS: Polymerase chain reaction (PCR) in combination with in situ hybridization was used to identify HPV-DNA in the patients' biopsies. The HPV typing was conducted by pyrosequencing. Cell cycle and DNA content were analysed by cytometry. RESULTS: Ninety percent of the carcinoma biopsies carried high-risk oncogenic HPV in their malignant cells. Eighty-one percent of these demonstrated a single infection with HPV16, 18 or 33 and 19% were double infected with HPV16 and HPV18. Accumulations of viral genes were seen at the necrotic area of the tumours. The HPV genome in the tumour cell influenced significantly the host cell cycle progression, but not DNA aberrations. Within these patients, HPVstatus in the malignant cells was not found to be associated with patient survival time. CONCLUSION: High-risk oncogenic HPV may play an important role in the initiation of host cell proliferation in anal squamous cell carcinoma. However, infection with HPV may not have any direct influence itself on the clinical outcome of these patients considering the treatments currently available.
Authors:
Nongnit Laytragoon-Lewin; Per J Nilsson; Juan Castro; Baback Gharizadeh; Pål Nyren; Bengt Glimelius; Göran Elmberger; Ingela Turesson; Christer Svensson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  27     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    2007 Nov-Dec
Date Detail:
Created Date:  2008-01-24     Completed Date:  2008-02-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  4473-9     Citation Subset:  IM    
Affiliation:
Department of Oncology, Rudbeck Laboratory, Uppsala University Hospital, Sweden. nongnit.laytragoon-lewin@onkologi.uu.se
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MeSH Terms
Descriptor/Qualifier:
Aged
Anus Neoplasms / genetics*,  mortality,  virology*
Carcinoma, Squamous Cell / genetics*,  mortality,  virology*
Cell Cycle
Chromosome Aberrations
DNA, Neoplasm / genetics*
Female
Humans
In Situ Hybridization
Kaplan-Meiers Estimate
Male
Papillomaviridae* / isolation & purification
Papillomavirus Infections / complications
Polymerase Chain Reaction
Prevalence
Tumor Virus Infections*
Chemical
Reg. No./Substance:
0/DNA, Neoplasm

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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