Document Detail


Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase.
MedLine Citation:
PMID:  832918     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An undifferentiated human pancreatic carcinoma has been established in continuous culture and is grown in Dulbecco's modified. Eagle's medium fortified with 10% fetal calf serum and 2.5% horse serum. The established cell line (MIA PaCa-2) has a doubling time of 40 h. The cells are large with abundant cytoplasm, exhibit a high degree of aneuploidy and have a tendency to grow on top of other cells. MIA PaCa-2 grows in soft agar with a colony-forming efficiency of 19%. Both MIA PaCa-2 cells and a cell line from another pancreatic carcinoma obtained from National Cancer Institute (NCI) are sensitive to asparaginase, a property not shared by several other human tumor cell lines tested.
Authors:
A A Yunis; G K Arimura; D J Russin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  19     ISSN:  0020-7136     ISO Abbreviation:  Int. J. Cancer     Publication Date:  1977 Jan 
Date Detail:
Created Date:  1977-03-15     Completed Date:  1977-03-15     Revised Date:  2007-07-24    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  128-35     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aged
Aneuploidy
Asparaginase / pharmacology*
Carmustine / pharmacology
Cell Division / drug effects
Cell Line
Cells, Cultured
Cytarabine / pharmacology
Fibrinolysis / drug effects
Humans
Male
Pancreatic Neoplasms* / genetics
Plasminogen / biosynthesis
Vincristine / pharmacology
Chemical
Reg. No./Substance:
147-94-4/Cytarabine; 154-93-8/Carmustine; 57-22-7/Vincristine; 9001-91-6/Plasminogen; EC 3.5.1.1/Asparaginase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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