Document Detail

Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase.
MedLine Citation:
PMID:  832918     Owner:  NLM     Status:  MEDLINE    
An undifferentiated human pancreatic carcinoma has been established in continuous culture and is grown in Dulbecco's modified. Eagle's medium fortified with 10% fetal calf serum and 2.5% horse serum. The established cell line (MIA PaCa-2) has a doubling time of 40 h. The cells are large with abundant cytoplasm, exhibit a high degree of aneuploidy and have a tendency to grow on top of other cells. MIA PaCa-2 grows in soft agar with a colony-forming efficiency of 19%. Both MIA PaCa-2 cells and a cell line from another pancreatic carcinoma obtained from National Cancer Institute (NCI) are sensitive to asparaginase, a property not shared by several other human tumor cell lines tested.
A A Yunis; G K Arimura; D J Russin
Related Documents :
20546238 - Differences between karyotypically normal and abnormal human embryonic stem cells.
9648098 - Cell line availability: where to get the cell lines you need.
1335788 - Oligonucleotide fingerprinting as a means to identify and survey long-term cultured b c...
2450098 - Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell...
20798818 - Competition-colonization dynamics: an ecology approach to quasispecies dynamics and vir...
23796248 - Differential effects of polyphenols on proliferation and apoptosis in human myeloid and...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  19     ISSN:  0020-7136     ISO Abbreviation:  Int. J. Cancer     Publication Date:  1977 Jan 
Date Detail:
Created Date:  1977-03-15     Completed Date:  1977-03-15     Revised Date:  2007-07-24    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  128-35     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Asparaginase / pharmacology*
Carmustine / pharmacology
Cell Division / drug effects
Cell Line
Cells, Cultured
Cytarabine / pharmacology
Fibrinolysis / drug effects
Pancreatic Neoplasms* / genetics
Plasminogen / biosynthesis
Vincristine / pharmacology
Reg. No./Substance:
147-94-4/Cytarabine; 154-93-8/Carmustine; 57-22-7/Vincristine; 9001-91-6/Plasminogen; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Immunoglobulin complexes in sera of patients with malignancy.
Next Document:  A dose-response study on urethane carcinogenesis in rats and mice.