Document Detail


Human oocyte vitrification: in-vivo and in-vitro maturation outcomes.
MedLine Citation:
PMID:  18983753     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study aimed to evaluate oocyte vitrification efficiency using in-vivo matured (IVO) versus rescued in-vitro matured (IVM) oocytes. The results show that oocyte survival (85% versus 81%), fertilization (86% versus 76%) and cleavage rate (98% versus 89%) was not significantly different in IVO oocytes compared with rescued IVM sibling oocytes. The fertilized oocytes from IVO and IVM groups were cultured to blastocyst stage; however, embryo development was significantly reduced in the rescued IVM group (72% versus 15%). Embryo transfer was only performed with the embryos derived from IVO oocytes on day 5; 42 blastocysts were transferred to 18 recipients; 16 of 18 recipients had positive beta-human chorionic gonadotrophin and a total of 26 fetal cardiac activities were detected in 15 recipients (implantation: 26/42, 61.9%). Ten of the 15 recipients have delivered 19 healthy babies, and the other five pregnancies are still ongoing. These data indicate that the combination of oocyte vitrification and rescued IVM not only yield a new strategy to extend the pool of total fertilizable oocytes, but also demonstrate that the efficiency of vitrified/warmed oocytes can be comparable to fresh oocytes with regard to clinical outcomes.
Authors:
Ching-Chien Chang; Daniel B Shapiro; Diana Patricia Bernal; Graham Wright; Hilton I Kort; Zsolt Peter Nagy
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  17     ISSN:  1472-6491     ISO Abbreviation:  Reprod. Biomed. Online     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-11-05     Completed Date:  2009-01-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  England    
Other Details:
Languages:  eng     Pagination:  684-8     Citation Subset:  IM    
Affiliation:
Reproductive Biology Associates, Atlanta, Georgia 30342, USA.
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MeSH Terms
Descriptor/Qualifier:
Adult
Blastocyst / cytology
Cleavage Stage, Ovum / cytology
Cryopreservation / methods*
Embryo Culture Techniques
Embryonic Development
Female
Fertilization in Vitro
Humans
Infant, Newborn
Oocytes / cytology,  growth & development*
Pregnancy
Pregnancy Outcome

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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