Document Detail

Human oocyte cryopreservation and the fate of cortical granules.
MedLine Citation:
PMID:  16756978     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To examine the effect of the commonly used oocyte cryopreservation protocol on the cortical granules (CGs) of human immature germinal vesicle (GV) and mature metaphase II (MII) oocytes. DESIGN: Laboratory study. SETTING: IVF unit. INTERVENTION(S): Unfertilized, intracytoplasmic sperm injected (ICSI) oocytes, and immature oocytes were cryopreserved using a slow freezing-rapid thawing program with 1,2-propanediol (PROH) as a cryoprotectant. MAIN OUTCOME MEASURE(S): Cortical granule exocytosis (CGE) was assessed by either confocal microscopy or transmission electron microscopy (TEM). RESULT(S): The survival rates of frozen-thawed oocytes (mature and immature) were significantly lower compared with zygotes. Both mature and immature oocytes exhibited increased fluorescence after cryopreservation, indicating the occurrence of CGE. Mere exposure of oocytes to cryoprotectants induced CGE of 70% the value of control zygotes. The TEM revealed a drastic reduction in the amount of CGs at the cortex of frozen-thawed GV and MII oocytes, as well as appearance of vesicles in the ooplasm. CONCLUSION(S): The commonly used PROH freezing protocol for human oocytes resulted in extensive CGE. This finding explains why ICSI is needed to achieve fertilization of frozen-thawed human oocytes.
Yehudith Ghetler; Ehud Skutelsky; Isaac Ben Nun; Liah Ben Dor; Dina Amihai; Ruth Shalgi
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Publication Detail:
Type:  Journal Article     Date:  2006-06-06
Journal Detail:
Title:  Fertility and sterility     Volume:  86     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-07-04     Completed Date:  2006-08-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  210-6     Citation Subset:  IM    
Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
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MeSH Terms
Cells, Cultured
Cryopreservation / methods*
Cytoplasmic Granules / physiology*,  ultrastructure*
Exocytosis / physiology*
Oocytes / cytology*,  physiology*

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